Differentially expressed genes in silkworm cell cultures in response to infection by Wolbachia and Cardinium endosymbionts

Y. Nakamura, T. Gotoh, S. Imanishi, K. Mita, T. J. Kurtti, Hiroaki Noda

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Wolbachia and Cardinium are bacterial endosymbionts that are widely distributed amongst arthropods. Both cause reproductive alterations, such as cytoplasmic incompatibility, parthenogenesis and feminization. Here we studied differentially expressed genes in Wolbachia- and Cardinium-infected Bm-aff3 silkworm cells using a silkworm microarray. Wolbachia infection did not alter gene expression or induce or suppress immune responses. In contrast, Cardinium infection induced many immune-related genes, including antimicrobial peptides, pattern recognition receptors and a serine protease. Host immune responses differed, possibly because of the different cell wall structures of Wolbachia and Cardinium because the former lacks genes encoding lipopolysaccharide components and two racemases for peptidoglycan formation. A few possibly non-immune-related genes were differentially expressed, but their involvement in host reproductive alteration was unclear.

Original languageEnglish (US)
Pages (from-to)279-289
Number of pages11
JournalInsect molecular biology
Volume20
Issue number3
DOIs
StatePublished - Jun 1 2011

Keywords

  • Cardinium
  • Gene expression
  • Microarray
  • Silkworm cell
  • Wolbachia

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