Differential responses from seven mammalian cell lines to the treatments of detoxifying enzyme inducers

Zhi Qiang Jiang, Chi Chen, Bo Yang, Vidya Hebbar, A. N.Tony Kong

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Cell-based models have been used extensively in screening novel bioactive chemical entities. In this study, seven well-established mammalian cell lines, which have different origins, were utilized to compare their responses to the treatments of three detoxifying enzyme inducers, tert-butylhydroquinone (tBHQ), β-naphthoflavone (β-NF), and sulforaphane (SUL), which are potential chemopreventive compounds. The enzymatic activities of glutathione s-transferase (GST), NAD(P)H:quinone oxidoreductase (QR), aldehyde reductase (AR), and glutathione reductase (GR) were measured by kinetics methods using UV-Vis spectroscopy, and analyzed statistically by Student's t-test. Among these mammalian cell lines, the mouse hepatoma Hepa1c1c7 cells were the most robust and sensitive cells, which had higher basal as well as upregulated enzymatic activities. In human cell lines, the prostate LNCaP and hepatic HepG2 cells were also very responsive to the inducers. The results suggested that different cell lines responded differently to individual detoxifying gene inducer, and the selection of appropriate cell line is important for screening potential chemopreventive agents.

Original languageEnglish (US)
Pages (from-to)2243-2253
Number of pages11
JournalLife Sciences
Volume72
Issue number20
DOIs
StatePublished - Apr 4 2003

Bibliographical note

Funding Information:
This work was supported in part by NIH grants (R01-CA73674 and R01-CA94828).

Keywords

  • AR
  • GR
  • GST
  • HT-29
  • HeLa
  • HepG2
  • Hepa1c1c7
  • LNCaP
  • MCF7
  • MDA-MB-231
  • QR
  • Sulforaphane
  • tBHQ
  • β-NF

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