Differential regulation of JAMM domain deubiquitinating enzyme activity within the RAP80 complex

Jeffrey Patterson-Fortin, Genze Shao, Heidi Bretscher, Troy E. Messick, Roger A. Greenberg

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

BRCC36 is a JAMM (JAB1/MPN/Mov34 metalloenzyme) domain, lysine 63-ubiquitin (K63-Ub)-specific deubiquitinating enzyme (DUB) and a member of two protein complexes: the DNA damage-responsive BRCA1-RAP80 complex, and the cytoplasmic BRCC36 isopeptidase complex (BRISC). The presence of several identical constituents in both complexes suggests common regulatory mechanisms and potential competition between K63-Ub-related signaling in cytoplasmic and nuclear compartments. Surprisingly, we discover that BRCC36 DUB activity requires different interactions within the context of each complex. Abraxas and BRCC45 were essential for BRCC36 DUB activity within the RAP80 complex, whereas KIAA0157/Abro was the only interaction required for DUB activity within the BRISC. Poh1 also required protein interactions for activity, suggesting a common regulatory mechanism for JAMM domain DUBs. Finally, BRISC deficiency enhanced formation of the BRCA1-RAP80 complex in vivo, increasing BRCA1 levels at DNA double strand breaks. These findings reveal that JAMM domain DUB activity and K63-Ub levels are regulated by multiple mechanisms within the cell.

Original languageEnglish (US)
Pages (from-to)30971-30981
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number40
DOIs
StatePublished - Oct 1 2010

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