Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [14C] N-ethyl maleimide

Gary Gardner; William F. Thompson; Winslow R. Briggs

doi:10.1007/BF00388032

Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [¹⁴C] N-ethyl maleimide

Gary Gardner, William F. Thompson, Winslow R. Briggs

Horticultural Science

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The red-absorbing form (P_r) and the far-red absorbing form (P_fr) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[¹⁴C]maleimide ([¹⁴C]-NEM). After pre-treatment of P_r with cold NEM and extensive dialysis, photoconversion to P_fr and treatment with [¹⁴C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.

Original language	English (US)
Pages (from-to)	367-372
Number of pages	6
Journal	Planta
Volume	117
Issue number	4
DOIs	https://doi.org/10.1007/BF00388032
State	Published - Dec 1974

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title = "Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [14C] N-ethyl maleimide",

abstract = "The red-absorbing form (Pr) and the far-red absorbing form (Pfr) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[14C]maleimide ([14C]-NEM). After pre-treatment of Pr with cold NEM and extensive dialysis, photoconversion to Pfr and treatment with [14C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.",

author = "Gary Gardner and Thompson, {William F.} and Briggs, {Winslow R.}",

year = "1974",

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doi = "10.1007/BF00388032",

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volume = "117",

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T1 - Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [14C] N-ethyl maleimide

AU - Gardner, Gary

AU - Thompson, William F.

AU - Briggs, Winslow R.

PY - 1974/12

Y1 - 1974/12

N2 - The red-absorbing form (Pr) and the far-red absorbing form (Pfr) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[14C]maleimide ([14C]-NEM). After pre-treatment of Pr with cold NEM and extensive dialysis, photoconversion to Pfr and treatment with [14C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.

AB - The red-absorbing form (Pr) and the far-red absorbing form (Pfr) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[14C]maleimide ([14C]-NEM). After pre-treatment of Pr with cold NEM and extensive dialysis, photoconversion to Pfr and treatment with [14C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.

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Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [¹⁴C] N-ethyl maleimide

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