Little is known about the participation of β chemokines in inflammatory processes within the central nervous system. The release of three of these peptides (macrophage inflammatory protein [MIP]-1α, MIP-1β, and monocyte chemoattractant protein-1) from human fetal microglial cell and astrocyte cultures was assessed following stimulation by lipopolysaccharide, interleukin-1β, and tumor necrosis factor-α. Although striking differences were found between these two types of glial cells in their responsiveness to lipopolysaccharide and cytokines, both microglia and astrocytes produced all three β chemokines. Only microglial cells, however, demonstrated an increased migratory response to the β chemokines. The results of this in vitro study suggest that β chemokines may play an important role in the trafficking of mononuclear phagocytes within the brain.
Bibliographical noteFunding Information:
Neuroimmunology and Host Defense Laboratory, Minneapolis Medical Research Foundation, and University ofMinnesota Medical School, Minneapolis, and College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota
Received 16 April 1996; revised 3 September 1996. Human brain tissue was obtained under a protocol approved by the institutional Human Subjects Research Committee. Informed consent was obtained for fetal brain tissue from patients who were undergoing elective abortions. Financial support: NIH (DA-04381 and DA-09924). Reprints or correspondence: Dr. Phillip K. Peterson, Dept. of Medicine, Hennepin County Medical Center, 701 Park Ave., Minneapolis, MN 55415.