Differential Induction of DNA-Binding Activities Following CD19 Cross-Linking in Human B Lineage Cells

Wen Kai Weng, Nisha Shah, Darin O'Brien, Brian G Van Ness, Tucker W LeBien

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The B cell-specific cell surface molecule CD19 is expressed at all stages of B cell development, including normal plasma cells, and mediates signal transduction via interaction with cytoplasmic effector proteins. Cross-linking CD19 on early human B lineage cells induces the formation of a CD19/Vav/phosphatidylinositol-3 kinase complex, tyrosine phosphorylation of CD19 and Vav, and activation of the Ras pathway. To further explore the ramifications of CD19 signaling, the current study examined whether phosphorylation of Elk-1, activation of activator protein-1 (AP-1), or activation of nuclear factor-κB (NF-κB) transcription factors occurred following CD19 cross-linking. The cells used were the BLIN-1 pre-B cell line expressing low levels of cell surface μ heavy chain associated with surrogate light chain and the 1E8 immature B cell line expressing cell surface μ/κ. Lysates from CD19 cross-linked 1E8 cells induced robust phosphorylation of an Elk-1 fusion protein in vitro, whereas no phosphorylation of Elk-1 fusion protein occurred using lysates from CD19 cross-linked BLIN-1 cells. An electrophoretic mobility shift assay employing AP-1 and NF-κB consensus oligonucleotides was used to demonstrate that AP-1 -binding activity increased, while constitutive NF-κB-binding activity was not enhanced, following 2 h of CD19 cross-linking in 1E8 cells. Supershift experiments revealed that JunD and c-Fos proteins mediated anti-CD19 induced AP-1-binding activity in 1E8 cells. In contrast, CD19 cross-linking in BLIN-1 cells resulted in the induction of NF-κB, but had no apparent effect on AP-1 -binding activity. These data suggest that CD19-mediated signal transduction activates different transcription factors at juxtaposed stages of B cell development that may culminate in the activation or suppression of distinct sets of genes.

Original languageEnglish (US)
Pages (from-to)5502-5508
Number of pages7
JournalJournal of Immunology
Issue number11
StatePublished - Dec 1 1997


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