We report and discuss the results of a quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of the expression patterns of six MADS-box genes (VsMB2 to VsMB7 genes) identified after Next Generation Sequencing (NGS) and assembly of two transcriptomes, those of the sporophyte and the gametophyte, of the endangered fern species Vandenboschia speciosa. Deduced VsMB amino acid sequences had a typical MIKCC-type structure consisting of a MADS domain, a short I region, a K domain, and a C-terminal region. Sequence and phylogenetic analyses supported the placement of the six analyzed proteins in the three main fern MIKCC protein subfamilies. The expression analyses of the six VsMB genes by qRT-PCR indicate that MIKCC-type genes have a broad expression in the sporophyte and that most of the VsMB genes appear to have also a broad expression in the gametophyte, an ancestral condition. However, two of the genes, VsMB2 and VsMB4, have a residual expression in the gametophyte. Furthermore, VsMB3 and VsMB7 genes are down-regulated in the reproductive structures. This study represents a comprehensive identification and characterization of MADS-box genes in V. speciosa and it supports specialized roles of some of these genes during the two phases of fern development.
Bibliographical noteFunding Information:
We thank Karl Meunier for language revision. This research has been financed by the Spanish Ministerio de Econom?a y Competitividad and FEDER funds, grant: CGL2010-14856 (subprograma BOS). The Consejer?a de Medio Ambiente y Ordenaci?n del Territorio de la Junta de Andaluc?a authorized and facilitates the sampling of the material. We are highly indebted to Javier Madrid Rojo (Director General de Gesti?n del Medio Natural y Espacios Protegidos), Fernando Ortega Alegre (Jefe de Servicio de Geodiversidad y Biodiversidad) and Carmen Rodr?guez Hiraldo (Jefa del Departamento de Conservaci?n de Flora y Hongos), and especially to Antonio J. Delgado V?zquez and to Jaime Pere?a Ortiz who, together the team of Agentes de Medio Ambiente of the Consejer?a, helped us with the sampling procedure. M. Bakkali wishes to thank the Spanish Ministerio de Econom?a y Competitividad for granting him with a Ram?n y Cajal Fellowship, the BFU2010-16438 grant, and an FPI studentship to R. Mart?n-Bl?zquez.
- Expression patterns
- MADS-box genes
- Vandenboschia speciosa