Differential expression of the two closely related LIM-Class homeobox genes LH-2A and LH-2B during limb development

Tsutomu Nohno, Yasuhiko Kawakami, Naoyuki Wada, Tetsuya Ishikawa, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We have isolated the chicken homeobox genes LH-2A and LH-2B encoding two related LIM domain-containing homeodomain proteins and examined the expression pattern during chick limb development. LH-2A is most closely related to human and rat LH-2, while LH-2B is less conserved. Although both LH-2A and LH-2B are expressed in the limb mesenchyme throughout stage 16 to stage 32, LH-2A transcripts are detectable in the distal limb bud and LH-2B transcripts are detectable in the anterior limb bud. Signals from the apical ectodermal ridge positively regulate LH-2A expression, since removal of the apical ectoderm resulted in the rapid reduction of LH-2A expression in the distal limb mesenchyme. Ectopic expression of the sonic hedgehog gene in the anterior margin of the limb bud resulted in the rapid reduction of LH-2B expression accompanying respecification of the positional value to the posterior phenotype. These results suggest that LH-2A and LH-2B play important roles in the determination and specification of the proximal-distal and anterior-posterior positional values, respectively.

Original languageEnglish (US)
Pages (from-to)506-511
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume238
Issue number2
DOIs
StatePublished - Sep 18 1997
Externally publishedYes

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