Differential expression of six glutamine synthetase genes in Zea mays

Min gang Li, Richard Villemur, Patrick J. Hussey, Carolyn D. Silflow, J. Stephen Gantt, D. Peter Snustad

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

The maize genome has been shown to contain six glutamine synthetase (GS) genes with at least four different expression patterns. Noncoding 3′ gene-specific probes were constructed from all six GS cDNA clones and used to examine transcript levels in selected organs by RNA gel blot hybridization experiments. The transcript of the single putative chloroplastic GS2 gene was found to accumulate primarily in green tissues, whereas the transcripts of the five putative GS1 genes were shown to accumulate preferentially in roots. The specific patterns of transcript accumulation were quite distinct for the five GS1 genes, with the exception of two closely related genes.

Original languageEnglish (US)
Pages (from-to)401-407
Number of pages7
JournalPlant molecular biology
Volume23
Issue number2
DOIs
StatePublished - Oct 1 1993

Keywords

  • Zea mays
  • gene-specific probes
  • glutamine synthetase
  • transcript accumulation

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