Differential expression of laminin isoforms in diabetic nephropathy and other renal diseases

Suman Setty, Alfred A. Michael, Alfred J. Fish, Michael Mauer, Ralph J. Butkowski, Ismo Virtanen, Youngki Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Laminin a non-collagenous glycoprotein is a major component of the renal glomerular basement membrane and mesangium. Thus far eleven distinct chains have been described, permutations of which make up 15 laminin isoforms. Laminin molecules interact with cells and other matrix molecules during organ development and differentiation. We studied the distribution of laminin isoforms in patients with type 1 diabetic nephropathy, membranous nephropathy, membranoproliferative glomerulonephritis and IgA nephropathy/ Henoch-Schönlein purpura. Immunofluorescence microscopic studies with laminin-chain-specific antibodies to the α1, α2, α5, β1, β2 and γ1 chains detected α2, β1 and γ1 chain expression in the normal mesangium and α5, β2 and γ1 in normal glomerular basement membrane. Significantly, constituents of the glomerular basement membrane, α5, β2 and γ1 chains were overexpressed in kidneys with diabetic nephropathy. Initially the constituents of the mesangium increased commensurate with the degree of mesangial expansion and degree of diabetic nephropathy. Reduction in α2 chain intensity was observed with severe mesangial expansion and in the areas of nodular glomerulosclerosis. In addition, with late disease aberrant expression of α2 and β2 chains was observed in the mesangium. Glomerular basement membrane in renal disease overexpressed molecules normally present in that location. In summary, the alterations in basement membrane composition in various renal diseases seem to not only reflect the balance between synthesis and degradation of normal basement membrane constituents, but also their aberrant expression.

Original languageEnglish (US)
Pages (from-to)859-868
Number of pages10
JournalModern Pathology
Volume25
Issue number6
DOIs
StatePublished - Jun 1 2012

Fingerprint

Diabetic Nephropathies
Laminin
Glomerular Basement Membrane
Protein Isoforms
Kidney
Basement Membrane
Membranoproliferative Glomerulonephritis
Schoenlein-Henoch Purpura
Membranous Glomerulonephritis
Immunoglobulin A
Fluorescent Antibody Technique
Glycoproteins
Antibodies

Keywords

  • diabetic nephropathy
  • isoforms
  • laminin

Cite this

Setty, S., Michael, A. A., Fish, A. J., Mauer, M., Butkowski, R. J., Virtanen, I., & Kim, Y. (2012). Differential expression of laminin isoforms in diabetic nephropathy and other renal diseases. Modern Pathology, 25(6), 859-868. https://doi.org/10.1038/modpathol.2011.216

Differential expression of laminin isoforms in diabetic nephropathy and other renal diseases. / Setty, Suman; Michael, Alfred A.; Fish, Alfred J.; Mauer, Michael; Butkowski, Ralph J.; Virtanen, Ismo; Kim, Youngki.

In: Modern Pathology, Vol. 25, No. 6, 01.06.2012, p. 859-868.

Research output: Contribution to journalArticle

Setty, S, Michael, AA, Fish, AJ, Mauer, M, Butkowski, RJ, Virtanen, I & Kim, Y 2012, 'Differential expression of laminin isoforms in diabetic nephropathy and other renal diseases', Modern Pathology, vol. 25, no. 6, pp. 859-868. https://doi.org/10.1038/modpathol.2011.216
Setty, Suman ; Michael, Alfred A. ; Fish, Alfred J. ; Mauer, Michael ; Butkowski, Ralph J. ; Virtanen, Ismo ; Kim, Youngki. / Differential expression of laminin isoforms in diabetic nephropathy and other renal diseases. In: Modern Pathology. 2012 ; Vol. 25, No. 6. pp. 859-868.
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