Differential expression of HLA-G and ILT-2 receptor in human tuberculosis: Localized versus disseminated disease

Abhinav Saurabh, Deepshi Thakral, Manish K. Mourya, Amar Singh, Anant Mohan, Anuj K. Bhatnagar, Dipendra K. Mitra, Uma Kanga

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4 Scopus citations

Abstract

Human leukocyte antigen-G (HLA-G) is an anti-inflammatory and immunosuppressive molecule that can modulate immune cell activation. The role of HLA-G in tuberculosis, an immune-mediated and chronic bacterial disease remains to be elucidated. We investigated the expression profile of soluble and membrane bound HLA-G in pulmonary TB (PTB), TB pleural effusion (TB-PE, localized disease) and Miliary TB (disseminated form). The expression of HLA-G receptor, ILT-2 was also determined on the immune cells. We observed that the plasma sHLA-G levels were significantly increased in Miliary TB than in TB-PE patients. In contrast, immunophenotyping revealed that the percent frequency of CD3+ T cells expressing HLA-G was significantly reduced in Miliary TB as compared to TB-PE, whereas frequency of CD14+ monocytes expressing HLA-G was significantly higher in TB-PE patients. Strikingly in the TB-PE cases, comparison of disease site, i.e. pleural effusion with peripheral blood showed increased expression of both soluble and surface HLA-G, whereas ILT-2 expressing cells were reduced at the local disease site. Furthermore, we demonstrated that in TB-PE cases, HLA-G expression on CD3+ T cells was influenced by broad spectrum MMP inhibitor. Thus, differential expression of HLA-G could potentially be a useful biomarker to distinguish different states of TB disease.

Original languageEnglish (US)
Pages (from-to)746-753
Number of pages8
JournalHuman Immunology
Volume77
Issue number9
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Keywords

  • HLA-G
  • ILT-2
  • Miliary tuberculosis
  • Tuberculosis-pleural effusion

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    Saurabh, A., Thakral, D., Mourya, M. K., Singh, A., Mohan, A., Bhatnagar, A. K., Mitra, D. K., & Kanga, U. (2016). Differential expression of HLA-G and ILT-2 receptor in human tuberculosis: Localized versus disseminated disease. Human Immunology, 77(9), 746-753. https://doi.org/10.1016/j.humimm.2016.01.004