Differential effects of morphine- and cocaine-induced nNOS immunoreactivity in the dentate gyrus of hippocampus of mice lacking μ-opioid receptors

Ji Hoon Yoo; Jae Han Cho; Seok Yong Lee; Sanggeol Lee; Horace H. Loh; Ing K. Ho; Choon Gon Jang

doi:10.1016/j.neulet.2005.10.089

Differential effects of morphine- and cocaine-induced nNOS immunoreactivity in the dentate gyrus of hippocampus of mice lacking μ-opioid receptors

Ji Hoon Yoo
, Jae Han Cho
, Seok Yong Lee
, Sanggeol Lee
, Horace H. Loh
, Ing K. Ho
, Choon Gon Jang

Pharmacology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

This study investigated the expression of nNOS after repeated morphine or cocaine administration in order to determine if nNOS (neuronal nitric oxide synthase) is involved in the morphine- or cocaine-induced behavioral sensitization in μ-opioid receptor knockout (MOR^-/-) mice. Higher numbers of nNOS-positive cells were observed in the dentate gyrus of the hippocampus (DG) of the wild-type (MOR^+/+) mice repeatedly treated with either morphine or cocaine than in the saline treated MOR^+/+ mice (morphine, +122%; cocaine, +82%). Moreover, the MOR^-/- mice also showed significantly higher morphine- or cocaine-induced nNOS expression levels in the DG than in the saline treated MOR^+/+ mice (morphine, +234%; cocaine, +54%). The MOR^-/- mice showed a significantly higher morphine-induced nNOS expression level (+103%) or a lower cocaine-induced nNOS expression level (+38%) in the DG than in the morphine- or cocaine-treated MOR^+/+ mice. These results suggest that morphine and cocaine sensitization is differentially regulated by the μ-opioid receptors in MOR^-/- mice via the nNOS systems in the DG.

Original language	English (US)
Pages (from-to)	98-102
Number of pages	5
Journal	Neuroscience Letters
Volume	395
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2005.10.089
State	Published - Mar 6 2006

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (2003-041-E00327) and Brain Research Center of the 21st Century Frontier Research Program (M103KV010008 05K2201 00830).

Keywords

Cocaine
Immunohistochemistry
Knockout mice
Morphine
nNOS
μ-Opioid receptors

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10.1016/j.neulet.2005.10.089

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title = "Differential effects of morphine- and cocaine-induced nNOS immunoreactivity in the dentate gyrus of hippocampus of mice lacking μ-opioid receptors",

abstract = "This study investigated the expression of nNOS after repeated morphine or cocaine administration in order to determine if nNOS (neuronal nitric oxide synthase) is involved in the morphine- or cocaine-induced behavioral sensitization in μ-opioid receptor knockout (MOR-/-) mice. Higher numbers of nNOS-positive cells were observed in the dentate gyrus of the hippocampus (DG) of the wild-type (MOR+/+) mice repeatedly treated with either morphine or cocaine than in the saline treated MOR+/+ mice (morphine, +122\%; cocaine, +82\%). Moreover, the MOR-/- mice also showed significantly higher morphine- or cocaine-induced nNOS expression levels in the DG than in the saline treated MOR+/+ mice (morphine, +234\%; cocaine, +54\%). The MOR-/- mice showed a significantly higher morphine-induced nNOS expression level (+103\%) or a lower cocaine-induced nNOS expression level (+38\%) in the DG than in the morphine- or cocaine-treated MOR+/+ mice. These results suggest that morphine and cocaine sensitization is differentially regulated by the μ-opioid receptors in MOR-/- mice via the nNOS systems in the DG.",

keywords = "Cocaine, Immunohistochemistry, Knockout mice, Morphine, nNOS, μ-Opioid receptors",

author = "Yoo, \{Ji Hoon\} and Cho, \{Jae Han\} and Lee, \{Seok Yong\} and Sanggeol Lee and Loh, \{Horace H.\} and Ho, \{Ing K.\} and Jang, \{Choon Gon\}",

note = "Funding Information: This work was supported by Korea Research Foundation Grant (2003-041-E00327) and Brain Research Center of the 21st Century Frontier Research Program (M103KV010008 05K2201 00830).",

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doi = "10.1016/j.neulet.2005.10.089",

language = "English (US)",

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T1 - Differential effects of morphine- and cocaine-induced nNOS immunoreactivity in the dentate gyrus of hippocampus of mice lacking μ-opioid receptors

AU - Yoo, Ji Hoon

AU - Cho, Jae Han

AU - Lee, Seok Yong

AU - Lee, Sanggeol

AU - Loh, Horace H.

AU - Ho, Ing K.

AU - Jang, Choon Gon

N1 - Funding Information: This work was supported by Korea Research Foundation Grant (2003-041-E00327) and Brain Research Center of the 21st Century Frontier Research Program (M103KV010008 05K2201 00830).

PY - 2006/3/6

Y1 - 2006/3/6

N2 - This study investigated the expression of nNOS after repeated morphine or cocaine administration in order to determine if nNOS (neuronal nitric oxide synthase) is involved in the morphine- or cocaine-induced behavioral sensitization in μ-opioid receptor knockout (MOR-/-) mice. Higher numbers of nNOS-positive cells were observed in the dentate gyrus of the hippocampus (DG) of the wild-type (MOR+/+) mice repeatedly treated with either morphine or cocaine than in the saline treated MOR+/+ mice (morphine, +122%; cocaine, +82%). Moreover, the MOR-/- mice also showed significantly higher morphine- or cocaine-induced nNOS expression levels in the DG than in the saline treated MOR+/+ mice (morphine, +234%; cocaine, +54%). The MOR-/- mice showed a significantly higher morphine-induced nNOS expression level (+103%) or a lower cocaine-induced nNOS expression level (+38%) in the DG than in the morphine- or cocaine-treated MOR+/+ mice. These results suggest that morphine and cocaine sensitization is differentially regulated by the μ-opioid receptors in MOR-/- mice via the nNOS systems in the DG.

AB - This study investigated the expression of nNOS after repeated morphine or cocaine administration in order to determine if nNOS (neuronal nitric oxide synthase) is involved in the morphine- or cocaine-induced behavioral sensitization in μ-opioid receptor knockout (MOR-/-) mice. Higher numbers of nNOS-positive cells were observed in the dentate gyrus of the hippocampus (DG) of the wild-type (MOR+/+) mice repeatedly treated with either morphine or cocaine than in the saline treated MOR+/+ mice (morphine, +122%; cocaine, +82%). Moreover, the MOR-/- mice also showed significantly higher morphine- or cocaine-induced nNOS expression levels in the DG than in the saline treated MOR+/+ mice (morphine, +234%; cocaine, +54%). The MOR-/- mice showed a significantly higher morphine-induced nNOS expression level (+103%) or a lower cocaine-induced nNOS expression level (+38%) in the DG than in the morphine- or cocaine-treated MOR+/+ mice. These results suggest that morphine and cocaine sensitization is differentially regulated by the μ-opioid receptors in MOR-/- mice via the nNOS systems in the DG.

KW - Cocaine

KW - Immunohistochemistry

KW - Knockout mice

KW - Morphine

KW - nNOS

KW - μ-Opioid receptors

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UR - https://www.scopus.com/pages/publications/31944438298#tab=citedBy

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SN - 0304-3940

VL - 395

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EP - 102

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Differential effects of morphine- and cocaine-induced nNOS immunoreactivity in the dentate gyrus of hippocampus of mice lacking μ-opioid receptors

Abstract

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