Differential coupling of m1, m3, and m5 muscarinic receptors to activation of neuronal nitric oxide synthase

Shou Zhen Wang, Seok Yong Lee, Sheng Zu Zhu, Esam E El-Fakahany

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The selectivity of coupling of m1, m3, and m5 muscarinic receptors to activation of the neuronal type of nitric oxide synthase was investigated. Stimulation with the agonist carbachol of all three receptor subtypes expressed in Chinese hamster ovary cells resulted in a rapid and transient activation of the enzyme, as measured by stimulation of guanylate cyclase in reporter neuroblastoma cells. Carbachol was more potent and efficacious at m5 receptors than at the other two receptor subtypes. Stimulation of all three muscarinic receptors resulted in an increased concentration of intracellular calcium, with a time course that preceded activation of nitric oxide synthase. At each receptor subtype, there was a close relationship between the magnitude of the maximal calcium response and that of enzyme activation.

Original languageEnglish (US)
Pages (from-to)271-280
Number of pages10
JournalPharmacology
Volume53
Issue number5
DOIs
StatePublished - 1996

Keywords

  • Calcium signaling
  • Guanylate cyclase
  • Muscarinic acetylcholine receptors
  • Nitric oxide synthase
  • Receptor subtypes

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