Differential activation of ascending noxious pathways associated with trigeminal nerve injury

Shinji Okada, Ayano Katagiri, Hiroto Saito, Jun Lee, Kinuyo Ohara, Toshimitsu Iinuma, David A. Bereiter, Koichi Iwata

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Trigeminal spinal subnucleus caudalis (Vc) neurons that project to the ventral posteromedial thalamic nucleus (VPM) and parabrachial nucleus (PBN) are critical for orofacial pain processing. We hypothesized that persistent trigeminal nerve injury differentially alters the proportion of Vc neurons that project to VPM and PBN in a modality-specific manner. Neuroanatomical approaches were used to quantify the number of Vc neurons projecting to VPM or PBN after chronic constriction injury of the infraorbital nerve (ION-CCI) and subsequent upper-lip stimulation. Male rats received injections of retrograde tracer fluorogold into the contralateral VPM or PBN on day 7 after ION-CCI, and at 3 days after that, either capsaicin injection or noxious mechanical stimulation was applied to the upper lip ipsilateral to nerve injury. Infraorbital nerve chronic constriction injury rats displayed greater forelimb wiping to capsaicin injection and mechanical allodynia of the lip than sham rats. Total cell counts for phosphorylated extracellular signal-regulated kinase-immunoreactive (pERK-IR) neurons after capsaicin or mechanical lip stimuli were higher in ION-CCI than sham rats as was the percentage of pERK-IR PBN projection neurons. However, the percentage of pERK-IR VPM projection neurons was also greater in ION-CCI than sham rats after capsaicin but not mechanical lip stimuli. The present findings suggest that persistent trigeminal nerve injury increases the number of Vc neurons activated by capsaicin or mechanical lip stimuli. By contrast, trigeminal nerve injury modifies the proportion of Vc nociceptive neurons projecting to VPM and PBN in a stimulus modality-specific manner and may reflect differential involvement of ascending pain pathways receiving C fiber and mechanosensitive afferents.

Original languageEnglish (US)
Pages (from-to)1342-1360
Number of pages19
JournalPain
Volume160
Issue number6
DOIs
StatePublished - Jun 1 2019

Bibliographical note

Funding Information:
This work was supported by the JSPS KAKENHI (Grant number 17K11855 to A.K.); in part by the Sato and Uemura Funds of Nihon University School of Dentistry; the Dental Research Center of Nihon University School of Dentistry; and National Institutes of Health Grant (EY028143 to D.A.B.). Author contributions: All authors read and approved the final manuscript. S. Okada and A. Katagiri performed the experiments and data analysis. H. Saito, J. Lee, K. Ohara, and T. Iinuma helped with the experiments and data analysis. A. Katagiri and K. Iwata conceptualized the hypothesis, designed and supervised the experiments, and directed the data analysis. A. Katagiri, D.A. Bereiter, and K. Iwata finalized the figures and text.

Publisher Copyright:
© 2019 International Association for the Study of Pain.

Keywords

  • Ascending noxious pathways
  • Nerve injury
  • Parabrachial nucleus
  • Trigeminal spinal subnucleus caudalis
  • Ventral posteromedial thalamic nucleus

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