Differences in platelet-rich plasma composition influence bone healing

Faez Saleh Al-Hamed; Lina Abu-Nada; Rania Rodan; Stylianos Sarrigiannidis; Jose Luis Ramirez-Garcialuna; Hanan Moussa; Osama Elkashty; Qiman Gao; Tayebeh Basiri; Laura Baca; Jesus Torres; Lisa Rancan; Simon D. Tran; Marie Lordkipanidzé; Mari Kaartinen; Zahi Badran; Faleh Tamimi

doi:10.1111/jcpe.13546

Differences in platelet-rich plasma composition influence bone healing

Faez Saleh Al-Hamed, Lina Abu-Nada, Rania Rodan, Stylianos Sarrigiannidis, Jose Luis Ramirez-Garcialuna, Hanan Moussa, Osama Elkashty, Qiman Gao, Tayebeh Basiri, Laura Baca, Jesus Torres, Lisa Rancan, Simon D. Tran, Marie Lordkipanidzé, Mari Kaartinen, Zahi Badran, Faleh Tamimi

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm³) compared to single-spin L-PRP (1.42 ± 0.27 mm³) or filtered L-PRP (1.38 ± 0.28 mm³). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.

Original language	English (US)
Pages (from-to)	1613-1623
Number of pages	11
Journal	Journal of clinical periodontology
Volume	48
Issue number	12
DOIs	https://doi.org/10.1111/jcpe.13546
State	Published - Dec 2021
Externally published	Yes

Bibliographical note

Funding Information:
Al Awn Foundation for Development, Yemen; Alpha Omega Foundation of Canada; Canada Research Chair Program; Funds de Recherche Québec ‐ Santé (FRQS: 257709); Le Réseau de recherche en santé buccodentaire et osseuse (RSBO) Funding information

Funding Information:
Faez Saleh Al‐Hamed was supported by a scholarship from Al Awn Foundation for Development, Yemen; Ph.D. training award from Funds de Recherche Québec—Santé (FRQS: 257709); and the Alpha Omega Foundation of Canada grant (2018 and 2019). He also received the Graduate Excellence Award from the Faculty of Dentistry, McGill University. Lina Abu‐Nada was supported by Ph.D. training award from Funds de Recherche Québec—Santé and RSBO. Marie Lordkipanidzé is a Canada Research Chair in Platelets as Vectors and Biomarkers. The authors also acknowledge support from the Canada Research Chair Program and Le Réseau de recherche en santé buccodentaire et osseuse (RSBO). Funding information

Publisher Copyright:
© 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.

Keywords

bone formation
bone healing
critical size defect
platelet concentrates
platelet-rich plasma
rat surgery

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1111/jcpe.13546

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Al-Hamed, F. S., Abu-Nada, L., Rodan, R., Sarrigiannidis, S., Ramirez-Garcialuna, J. L., Moussa, H., Elkashty, O., Gao, Q., Basiri, T., Baca, L., Torres, J., Rancan, L., Tran, S. D., Lordkipanidzé, M., Kaartinen, M., Badran, Z., & Tamimi, F. (2021). Differences in platelet-rich plasma composition influence bone healing. Journal of clinical periodontology, 48(12), 1613-1623. https://doi.org/10.1111/jcpe.13546

Al-Hamed, FS, Abu-Nada, L, Rodan, R, Sarrigiannidis, S, Ramirez-Garcialuna, JL, Moussa, H, Elkashty, O, Gao, Q, Basiri, T, Baca, L, Torres, J, Rancan, L, Tran, SD, Lordkipanidzé, M, Kaartinen, M, Badran, Z & Tamimi, F 2021, 'Differences in platelet-rich plasma composition influence bone healing', Journal of clinical periodontology, vol. 48, no. 12, pp. 1613-1623. https://doi.org/10.1111/jcpe.13546

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title = "Differences in platelet-rich plasma composition influence bone healing",

abstract = "Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.",

keywords = "bone formation, bone healing, critical size defect, platelet concentrates, platelet-rich plasma, rat surgery",

author = "Al-Hamed, {Faez Saleh} and Lina Abu-Nada and Rania Rodan and Stylianos Sarrigiannidis and Ramirez-Garcialuna, {Jose Luis} and Hanan Moussa and Osama Elkashty and Qiman Gao and Tayebeh Basiri and Laura Baca and Jesus Torres and Lisa Rancan and Tran, {Simon D.} and Marie Lordkipanidz{\'e} and Mari Kaartinen and Zahi Badran and Faleh Tamimi",

note = "Funding Information: Al Awn Foundation for Development, Yemen; Alpha Omega Foundation of Canada; Canada Research Chair Program; Funds de Recherche Qu{\'e}bec ‐ Sant{\'e} (FRQS: 257709); Le R{\'e}seau de recherche en sant{\'e} buccodentaire et osseuse (RSBO) Funding information Funding Information: Faez Saleh Al‐Hamed was supported by a scholarship from Al Awn Foundation for Development, Yemen; Ph.D. training award from Funds de Recherche Qu{\'e}bec—Sant{\'e} (FRQS: 257709); and the Alpha Omega Foundation of Canada grant (2018 and 2019). He also received the Graduate Excellence Award from the Faculty of Dentistry, McGill University. Lina Abu‐Nada was supported by Ph.D. training award from Funds de Recherche Qu{\'e}bec—Sant{\'e} and RSBO. Marie Lordkipanidz{\'e} is a Canada Research Chair in Platelets as Vectors and Biomarkers. The authors also acknowledge support from the Canada Research Chair Program and Le R{\'e}seau de recherche en sant{\'e} buccodentaire et osseuse (RSBO). Funding information Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.",

year = "2021",

month = dec,

doi = "10.1111/jcpe.13546",

language = "English (US)",

volume = "48",

pages = "1613--1623",

journal = "Journal of Clinical Periodontology",

issn = "0303-6979",

publisher = "Blackwell Munksgaard",

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TY - JOUR

T1 - Differences in platelet-rich plasma composition influence bone healing

AU - Al-Hamed, Faez Saleh

AU - Abu-Nada, Lina

AU - Rodan, Rania

AU - Sarrigiannidis, Stylianos

AU - Ramirez-Garcialuna, Jose Luis

AU - Moussa, Hanan

AU - Elkashty, Osama

AU - Gao, Qiman

AU - Basiri, Tayebeh

AU - Baca, Laura

AU - Torres, Jesus

AU - Rancan, Lisa

AU - Tran, Simon D.

AU - Lordkipanidzé, Marie

AU - Kaartinen, Mari

AU - Badran, Zahi

AU - Tamimi, Faleh

N1 - Funding Information: Al Awn Foundation for Development, Yemen; Alpha Omega Foundation of Canada; Canada Research Chair Program; Funds de Recherche Québec ‐ Santé (FRQS: 257709); Le Réseau de recherche en santé buccodentaire et osseuse (RSBO) Funding information Funding Information: Faez Saleh Al‐Hamed was supported by a scholarship from Al Awn Foundation for Development, Yemen; Ph.D. training award from Funds de Recherche Québec—Santé (FRQS: 257709); and the Alpha Omega Foundation of Canada grant (2018 and 2019). He also received the Graduate Excellence Award from the Faculty of Dentistry, McGill University. Lina Abu‐Nada was supported by Ph.D. training award from Funds de Recherche Québec—Santé and RSBO. Marie Lordkipanidzé is a Canada Research Chair in Platelets as Vectors and Biomarkers. The authors also acknowledge support from the Canada Research Chair Program and Le Réseau de recherche en santé buccodentaire et osseuse (RSBO). Funding information Publisher Copyright: © 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.

PY - 2021/12

Y1 - 2021/12

N2 - Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.

AB - Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.

KW - bone formation

KW - bone healing

KW - critical size defect

KW - platelet concentrates

KW - platelet-rich plasma

KW - rat surgery

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DO - 10.1111/jcpe.13546

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JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

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ER -

Differences in platelet-rich plasma composition influence bone healing

Abstract

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Keywords

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