Differences in platelet-rich plasma composition influence bone healing

Faez Saleh Al-Hamed, Lina Abu-Nada, Rania Rodan, Stylianos Sarrigiannidis, Jose Luis Ramirez-Garcialuna, Hanan Moussa, Osama Elkashty, Qiman Gao, Tayebeh Basiri, Laura Baca, Jesus Torres, Lisa Rancan, Simon D. Tran, Marie Lordkipanidzé, Mari Kaartinen, Zahi Badran, Faleh Tamimi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.

Original languageEnglish (US)
Pages (from-to)1613-1623
Number of pages11
JournalJournal of clinical periodontology
Volume48
Issue number12
DOIs
StatePublished - Dec 2021
Externally publishedYes

Bibliographical note

Funding Information:
Al Awn Foundation for Development, Yemen; Alpha Omega Foundation of Canada; Canada Research Chair Program; Funds de Recherche Québec ‐ Santé (FRQS: 257709); Le Réseau de recherche en santé buccodentaire et osseuse (RSBO) Funding information

Funding Information:
Faez Saleh Al‐Hamed was supported by a scholarship from Al Awn Foundation for Development, Yemen; Ph.D. training award from Funds de Recherche Québec—Santé (FRQS: 257709); and the Alpha Omega Foundation of Canada grant (2018 and 2019). He also received the Graduate Excellence Award from the Faculty of Dentistry, McGill University. Lina Abu‐Nada was supported by Ph.D. training award from Funds de Recherche Québec—Santé and RSBO. Marie Lordkipanidzé is a Canada Research Chair in Platelets as Vectors and Biomarkers. The authors also acknowledge support from the Canada Research Chair Program and Le Réseau de recherche en santé buccodentaire et osseuse (RSBO). Funding information

Publisher Copyright:
© 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.

Keywords

  • bone formation
  • bone healing
  • critical size defect
  • platelet concentrates
  • platelet-rich plasma
  • rat surgery

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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