Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation

A. H.Y. Yuen, Z. Khalpey, M. Lavitrano, C. G.A. McGregor, K. K. Kalsi, M. H. Yacoub, R. T. Smolenski

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5′nucleotidease (E5′N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.

Original languageEnglish (US)
Pages (from-to)1221-1224
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume25
Issue number9-11
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the Magdi Yacoub Institute. Address correspondence to R. T. Smolenski, Heart Science Centre, Imperial College at Harefield Hospital, Harefield UB9 6JH, UK. E-mail: r.smolenski@imperial.ac.uk

Keywords

  • Ecto-5′-nucleotidase
  • Purine metabolism
  • Xenotransplantation

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