Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation

A. H.Y. Yuen; Z. Khalpey; M. Lavitrano; C. G.A. McGregor; K. K. Kalsi; M. H. Yacoub; R. T. Smolenski

doi:10.1080/15257770600894204

Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation

A. H.Y. Yuen, Z. Khalpey, M. Lavitrano, C. G.A. McGregor, K. K. Kalsi, M. H. Yacoub, R. T. Smolenski

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5′nucleotidease (E5′N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.

Original language	English (US)
Pages (from-to)	1221-1224
Number of pages	4
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	25
Issue number	9-11
DOIs	https://doi.org/10.1080/15257770600894204
State	Published - Jun 1 2006
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Magdi Yacoub Institute. Address correspondence to R. T. Smolenski, Heart Science Centre, Imperial College at Harefield Hospital, Harefield UB9 6JH, UK. E-mail: [email protected]

Keywords

Ecto-5′-nucleotidase
Purine metabolism
Xenotransplantation

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10.1080/15257770600894204

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title = "Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation",

abstract = "Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5′nucleotidease (E5′N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.",

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author = "Yuen, {A. H.Y.} and Z. Khalpey and M. Lavitrano and McGregor, {C. G.A.} and Kalsi, {K. K.} and Yacoub, {M. H.} and Smolenski, {R. T.}",

note = "Funding Information: This study was supported by the Magdi Yacoub Institute. Address correspondence to R. T. Smolenski, Heart Science Centre, Imperial College at Harefield Hospital, Harefield UB9 6JH, UK. E-mail: [email protected]",

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AU - Yuen, A. H.Y.

AU - Khalpey, Z.

AU - Lavitrano, M.

AU - McGregor, C. G.A.

AU - Kalsi, K. K.

AU - Yacoub, M. H.

AU - Smolenski, R. T.

N1 - Funding Information: This study was supported by the Magdi Yacoub Institute. Address correspondence to R. T. Smolenski, Heart Science Centre, Imperial College at Harefield Hospital, Harefield UB9 6JH, UK. E-mail: [email protected]

PY - 2006/6/1

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N2 - Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5′nucleotidease (E5′N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.

AB - Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5′nucleotidease (E5′N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.

KW - Ecto-5′-nucleotidase

KW - Purine metabolism

KW - Xenotransplantation

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Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation

Abstract

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