Dietary Zinc Supplemented in Organic Form Affects the Expression of Inflammatory Molecules in Swine Intestine

Ramya Lekha Medida, Ashok Kumar Sharma, Yue Guo, Lee J Johnston, Pedro E. Urriola, Andres M Gomez Zapata, Milena Saqui-Salces

Research output: Contribution to journalArticlepeer-review


Animals receiving Zinc (Zn) dietary supplementation with organic sources respond better to stress than inorganic Zn sources supplementation. The study aimed to identify the effect of different Zn sources on intestinal epithelial gene expression. In total, 45 pigs (9 per treatment) (77.5 ± 2.5 kg weight) were fed for 32 days, a corn-soybean meal diet without supplemented Zn (ZnR) or supplemented with 50 and 100 ppm of inorganic ZnCl2 (Zn50 and Zn100), and amino acid-bound organic Zn sources (LQ50 and LQ100). Gene expression changes form RNA-seq in ileum tissues of ZnR revealed changes associated with Zn insufficiency. Comparing organic with inorganic Zn sources by one-way ANOVA, pro-inflammatory cytokine interleukin 18 (IL18) was downregulated (p = 0.03) and Toll-like receptor 2 (TLR2) upregulated (p = 0.02). To determine the role of epithelial cells in response to dietary Zn, swine intestinal organoids (enteroids) were exposed to Zn restriction, ZnCl2 or LQ-Zn. In enteroids, ZIP4 expression decreased with added Zn compared with Zn-restriction (p = 0.006) but Zn sources did not affect (p > 0.05) IL18 or TLR2 expression. These results suggest that organic Zn may stimulate TLR2 signaling possibly affecting immune response, while decreasing the proinflammatory cytokine IL18 expression in non-epithelial cells of intestinal mucosa.

Original languageEnglish (US)
Article number2519
Issue number15
StatePublished - Aug 2023

Bibliographical note

Funding Information:
This research was partially supported by the Minnesota Experimental Station (MAES) project MIN-16-112 to M.S.-S.

Publisher Copyright:
© 2023 by the authors.


  • immune response
  • organic zinc
  • organoids
  • zinc sources

PubMed: MeSH publication types

  • Journal Article


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