Dietary deficiency of antioxidants exacerbates ischemic injury in the rat kidney

Karl A. Nath, Mark S. Paller

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71 Scopus citations


We examined the effects of dietary deficiency of vitamin E and selenium on the ischemia-reperfusion model of renal injury in the rat. Deficient diets imposed for six weeks on three-week-old weanling rats led to no significant differences in body weights, serum creatinine, GFR, RBF, TmPAH or urinary total protein excretory rates prior to ischemia. Twenty-four hours after one hour of ischemia, animals on the deficient diet demonstrated more markedly impaired GFR, RBF, TmPAH and urine to plasma creatinine concentrations and an increased renal failure index. Tubular damage was more severe injury in the deficient animals. Lipid peroxidation, 15 minutes after the release of the ischemic clamp, was increased in the deficient animals. We confirmed the effects of our dietary manipulation in impairing the oxidant scavenging system in the deficient animals since glutathione peroxidase activity was reduced to less than 5% in the basal state, and this striking reduction persisted following ischemia. Plasma vitamin E concentrations were also markedly depressed in the deficient diets. This dietary deficiency also worsened the course of acute renal injury and was accompanied by 50% mortality compared to 0% mortality in the control animals. Thus, dietary deficiency of vitamin E and selenium led to greater structural and functional renal impairment and increased lipid peroxidation following ischemia. These data provide support for the role of reactive oxygen species in mediating ischemia-reperfusion injury.

Original languageEnglish (US)
Pages (from-to)1109-1117
Number of pages9
JournalKidney international
Issue number6
StatePublished - Dec 1990

Bibliographical note

Funding Information:
We thank Rita Suck for assistance in preparing the manuscript. Funding for this study was provided by the NIH grant R29-DK38767 (First Award) to KAN and NIH grant HL-l7871 to MSP. Portions of this work were presented at the American Society of Nephrology annual meeting on December, 1988 in San Antonio, Texas, and were


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