Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Hamid A. Bakshi; Mazhar S.Al Zoubi; Hakkim L. Faruck; Alaa A.A. Aljabali; Firas A. Rabi; Amin A. Hafiz; Khalid M. Al-Batanyeh; Bahaa Al-Trad; Prawej Ansari; Mohamed M. Nasef; Nitin B. Charbe; Saurabh Satija; Meenu Mehta; Vijay Mishra; Gaurav Gupta; Salem Abobaker; Poonam Negi; Ibrahim M. Azzouz; Ashref Ali K. Dardouri; Harish Dureja; Parteek Prasher; Dinesh K. Chellappan; Kamal Dua; Mateus Webba Da Silva; Mohamed El Tanani; Paul A. McCarron; Murtaza M. Tambuwala

doi:10.3390/nu12061901

Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Hamid A. Bakshi
, Mazhar S.Al Zoubi
, Hakkim L. Faruck
, Alaa A.A. Aljabali
, Firas A. Rabi
, Amin A. Hafiz
, Khalid M. Al-Batanyeh
, Bahaa Al-Trad
, Prawej Ansari
, Mohamed M. Nasef
, Nitin B. Charbe
, Saurabh Satija
, Meenu Mehta
, Vijay Mishra
, Gaurav Gupta
, Salem Abobaker
, Poonam Negi
, Ibrahim M. Azzouz
, Ashref Ali K. Dardouri
, Harish Dureja

Parteek Prasher, Dinesh K. Chellappan, Kamal Dua, Mateus Webba Da Silva, Mohamed El Tanani, Paul A. McCarron, Murtaza M. Tambuwala

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

Original language	English (US)
Article number	1901
Pages (from-to)	1-19
Number of pages	19
Journal	Nutrients
Volume	12
Issue number	6
DOIs	https://doi.org/10.3390/nu12061901 https://doi.org/10.3390/nu12061901
State	Published - Jun 26 2020
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT_NUMBER: #295).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Cell cycle
Crocin
Hepatic injury
Pancreatic cancer
Radiation

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Bakshi, H. A., Zoubi, M. S. A., Faruck, H. L., Aljabali, A. A. A., Rabi, F. A., Hafiz, A. A., Al-Batanyeh, K. M., Al-Trad, B., Ansari, P., Nasef, M. M., Charbe, N. B., Satija, S., Mehta, M., Mishra, V., Gupta, G., Abobaker, S., Negi, P., Azzouz, I. M., Dardouri, A. A. K., ... Tambuwala, M. M. (2020). Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage. Nutrients, 12(6), 1-19. Article 1901. https://doi.org/10.3390/nu12061901, https://doi.org/10.3390/nu12061901

Bakshi, HA, Zoubi, MSA, Faruck, HL, Aljabali, AAA, Rabi, FA, Hafiz, AA, Al-Batanyeh, KM, Al-Trad, B, Ansari, P, Nasef, MM, Charbe, NB, Satija, S, Mehta, M, Mishra, V, Gupta, G, Abobaker, S, Negi, P, Azzouz, IM, Dardouri, AAK, Dureja, H, Prasher, P, Chellappan, DK, Dua, K, Da Silva, MW, Tanani, ME, McCarron, PA & Tambuwala, MM 2020, 'Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage', Nutrients, vol. 12, no. 6, 1901, pp. 1-19. https://doi.org/10.3390/nu12061901, https://doi.org/10.3390/nu12061901

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title = "Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage",

abstract = "Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.",

keywords = "Apoptosis, Cell cycle, Crocin, Hepatic injury, Pancreatic cancer, Radiation",

author = "Bakshi, \{Hamid A.\} and Zoubi, \{Mazhar S.Al\} and Faruck, \{Hakkim L.\} and Aljabali, \{Alaa A.A.\} and Rabi, \{Firas A.\} and Hafiz, \{Amin A.\} and Al-Batanyeh, \{Khalid M.\} and Bahaa Al-Trad and Prawej Ansari and Nasef, \{Mohamed M.\} and Charbe, \{Nitin B.\} and Saurabh Satija and Meenu Mehta and Vijay Mishra and Gaurav Gupta and Salem Abobaker and Poonam Negi and Azzouz, \{Ibrahim M.\} and Dardouri, \{Ashref Ali K.\} and Harish Dureja and Parteek Prasher and Chellappan, \{Dinesh K.\} and Kamal Dua and \{Da Silva\}, \{Mateus Webba\} and Tanani, \{Mohamed El\} and McCarron, \{Paul A.\} and Tambuwala, \{Murtaza M.\}",

note = "Funding Information: Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT\_NUMBER: \#295). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = jun,

day = "26",

doi = "10.3390/nu12061901",

language = "English (US)",

volume = "12",

pages = "1--19",

journal = "Nutrients",

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T1 - Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

AU - Bakshi, Hamid A.

AU - Zoubi, Mazhar S.Al

AU - Faruck, Hakkim L.

AU - Aljabali, Alaa A.A.

AU - Rabi, Firas A.

AU - Hafiz, Amin A.

AU - Al-Batanyeh, Khalid M.

AU - Al-Trad, Bahaa

AU - Ansari, Prawej

AU - Nasef, Mohamed M.

AU - Charbe, Nitin B.

AU - Satija, Saurabh

AU - Mehta, Meenu

AU - Mishra, Vijay

AU - Gupta, Gaurav

AU - Abobaker, Salem

AU - Negi, Poonam

AU - Azzouz, Ibrahim M.

AU - Dardouri, Ashref Ali K.

AU - Dureja, Harish

AU - Prasher, Parteek

AU - Chellappan, Dinesh K.

AU - Dua, Kamal

AU - Da Silva, Mateus Webba

AU - Tanani, Mohamed El

AU - McCarron, Paul A.

AU - Tambuwala, Murtaza M.

N1 - Funding Information: Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT_NUMBER: #295). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/6/26

Y1 - 2020/6/26

N2 - Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

AB - Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

KW - Apoptosis

KW - Cell cycle

KW - Crocin

KW - Hepatic injury

KW - Pancreatic cancer

KW - Radiation

UR - https://www.scopus.com/pages/publications/85087398483

UR - https://www.scopus.com/pages/publications/85087398483#tab=citedBy

U2 - 10.3390/nu12061901

DO - 10.3390/nu12061901

M3 - Article

C2 - 32604971

AN - SCOPUS:85087398483

SN - 2072-6643

VL - 12

SP - 1

EP - 19

JO - Nutrients

JF - Nutrients

IS - 6

M1 - 1901

ER -

Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Abstract

Bibliographical note

UN SDGs

Keywords

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