Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Hamid A. Bakshi; Mazhar S.Al Zoubi; Hakkim L. Faruck; Alaa A.A. Aljabali; Firas A. Rabi; Amin A. Hafiz; Khalid M. Al-Batanyeh; Bahaa Al-Trad; Prawej Ansari; Mohamed M. Nasef; Nitin B. Charbe; Saurabh Satija; Meenu Mehta; Vijay Mishra; Gaurav Gupta; Salem Abobaker; Poonam Negi; Ibrahim M. Azzouz; Ashref Ali K. Dardouri; Harish Dureja; Parteek Prasher; Dinesh K. Chellappan; Kamal Dua; Mateus Webba Da Silva; Mohamed El Tanani; Paul A. McCarron; Murtaza M. Tambuwala

doi:10.3390/nu12061901

Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Hamid A. Bakshi, Mazhar S.Al Zoubi, Hakkim L. Faruck, Alaa A.A. Aljabali, Firas A. Rabi, Amin A. Hafiz, Khalid M. Al-Batanyeh, Bahaa Al-Trad, Prawej Ansari, Mohamed M. Nasef, Nitin B. Charbe, Saurabh Satija, Meenu Mehta, Vijay Mishra, Gaurav Gupta, Salem Abobaker, Poonam Negi, Ibrahim M. Azzouz, Ashref Ali K. Dardouri, Harish DurejaParteek Prasher, Dinesh K. Chellappan, Kamal Dua, Mateus Webba Da Silva, Mohamed El Tanani, Paul A. McCarron, Murtaza M. Tambuwala

Research output: Contribution to journal › Article › peer-review

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Abstract

Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

Original language	English (US)
Article number	1901
Pages (from-to)	1-19
Number of pages	19
Journal	Nutrients
Volume	12
Issue number	6
DOIs	https://doi.org/10.3390/nu12061901 https://doi.org/10.3390/nu12061901
State	Published - Jun 26 2020
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT_NUMBER: #295).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Apoptosis
Cell cycle
Crocin
Hepatic injury
Pancreatic cancer
Radiation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Bakshi, H. A., Zoubi, M. S. A., Faruck, H. L., Aljabali, A. A. A., Rabi, F. A., Hafiz, A. A., Al-Batanyeh, K. M., Al-Trad, B., Ansari, P., Nasef, M. M., Charbe, N. B., Satija, S., Mehta, M., Mishra, V., Gupta, G., Abobaker, S., Negi, P., Azzouz, I. M., Dardouri, A. A. K., ... Tambuwala, M. M. (2020). Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage. Nutrients, 12(6), 1-19. Article 1901. https://doi.org/10.3390/nu12061901, https://doi.org/10.3390/nu12061901

Bakshi, HA, Zoubi, MSA, Faruck, HL, Aljabali, AAA, Rabi, FA, Hafiz, AA, Al-Batanyeh, KM, Al-Trad, B, Ansari, P, Nasef, MM, Charbe, NB, Satija, S, Mehta, M, Mishra, V, Gupta, G, Abobaker, S, Negi, P, Azzouz, IM, Dardouri, AAK, Dureja, H, Prasher, P, Chellappan, DK, Dua, K, Da Silva, MW, Tanani, ME, McCarron, PA & Tambuwala, MM 2020, 'Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage', Nutrients, vol. 12, no. 6, 1901, pp. 1-19. https://doi.org/10.3390/nu12061901, https://doi.org/10.3390/nu12061901

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title = "Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage",

abstract = "Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.",

keywords = "Apoptosis, Cell cycle, Crocin, Hepatic injury, Pancreatic cancer, Radiation",

author = "Bakshi, {Hamid A.} and Zoubi, {Mazhar S.Al} and Faruck, {Hakkim L.} and Aljabali, {Alaa A.A.} and Rabi, {Firas A.} and Hafiz, {Amin A.} and Al-Batanyeh, {Khalid M.} and Bahaa Al-Trad and Prawej Ansari and Nasef, {Mohamed M.} and Charbe, {Nitin B.} and Saurabh Satija and Meenu Mehta and Vijay Mishra and Gaurav Gupta and Salem Abobaker and Poonam Negi and Azzouz, {Ibrahim M.} and Dardouri, {Ashref Ali K.} and Harish Dureja and Parteek Prasher and Chellappan, {Dinesh K.} and Kamal Dua and {Da Silva}, {Mateus Webba} and Tanani, {Mohamed El} and McCarron, {Paul A.} and Tambuwala, {Murtaza M.}",

note = "Funding Information: Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT_NUMBER: #295). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = jun,

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doi = "10.3390/nu12061901",

language = "English (US)",

volume = "12",

pages = "1--19",

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AU - Bakshi, Hamid A.

AU - Zoubi, Mazhar S.Al

AU - Faruck, Hakkim L.

AU - Aljabali, Alaa A.A.

AU - Rabi, Firas A.

AU - Hafiz, Amin A.

AU - Al-Batanyeh, Khalid M.

AU - Al-Trad, Bahaa

AU - Ansari, Prawej

AU - Nasef, Mohamed M.

AU - Charbe, Nitin B.

AU - Satija, Saurabh

AU - Mehta, Meenu

AU - Mishra, Vijay

AU - Gupta, Gaurav

AU - Abobaker, Salem

AU - Negi, Poonam

AU - Azzouz, Ibrahim M.

AU - Dardouri, Ashref Ali K.

AU - Dureja, Harish

AU - Prasher, Parteek

AU - Chellappan, Dinesh K.

AU - Dua, Kamal

AU - Da Silva, Mateus Webba

AU - Tanani, Mohamed El

AU - McCarron, Paul A.

AU - Tambuwala, Murtaza M.

N1 - Funding Information: Acknowledgements: Authors are highly thankful to Dr. Smitha Sam (Clinical Oncologist), Director St. Jude institute of medical sciences and research center, for providing facilities for conducting in vivo studies Funding: 1. Authors are grateful to M.P Council of Science and Technology for providing young scientist award grant to Hamid A. Bakshi (MPCST/YSFA/27/2012). 2. Authors are grateful to Dowager Countess Eleanor Peel Trust (Liverpool) for funding awarded to Murtaza Tambuwala (GRANT_NUMBER: #295). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/6/26

Y1 - 2020/6/26

N2 - Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

AB - Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.

KW - Apoptosis

KW - Cell cycle

KW - Crocin

KW - Hepatic injury

KW - Pancreatic cancer

KW - Radiation

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M3 - Article

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SN - 2072-6643

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JO - Nutrients

JF - Nutrients

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M1 - 1901

ER -

Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Abstract

Bibliographical note

Keywords

UN SDGs

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