Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs

Ping Liu, Jinbiao Zhao, Pingting Guo, Wenqing Lu, Zhengying Geng, Crystal L. Levesque, Lee J. Johnston, Chunlin Wang, Ling Liu, Jie Zhang, Ning Ma, Shiyan Qiao, Xi Ma

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.

Original languageEnglish (US)
Article number526
JournalFrontiers in Cellular and Infection Microbiology
Volume7
Issue numberDEC
DOIs
StatePublished - Dec 22 2017

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Microbiota
Bacillus subtilis
Zea mays
Swine
Bacteria
Growth
Diet
Fibrobacteres
Dietary Fiber
rRNA Genes
Population
Fermentation
Food
Control Groups

Keywords

  • Bacillus subtilis
  • Cellulolytic bacteria
  • Corn bran
  • Dietary fiber
  • Fermentation
  • Gut microbiota

Cite this

Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs. / Liu, Ping; Zhao, Jinbiao; Guo, Pingting; Lu, Wenqing; Geng, Zhengying; Levesque, Crystal L.; Johnston, Lee J.; Wang, Chunlin; Liu, Ling; Zhang, Jie; Ma, Ning; Qiao, Shiyan; Ma, Xi.

In: Frontiers in Cellular and Infection Microbiology, Vol. 7, No. DEC, 526, 22.12.2017.

Research output: Contribution to journalArticle

Liu, Ping ; Zhao, Jinbiao ; Guo, Pingting ; Lu, Wenqing ; Geng, Zhengying ; Levesque, Crystal L. ; Johnston, Lee J. ; Wang, Chunlin ; Liu, Ling ; Zhang, Jie ; Ma, Ning ; Qiao, Shiyan ; Ma, Xi. / Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs. In: Frontiers in Cellular and Infection Microbiology. 2017 ; Vol. 7, No. DEC.
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abstract = "Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10{\%} CB diet, and a 10{\%} fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.",
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AU - Zhao, Jinbiao

AU - Guo, Pingting

AU - Lu, Wenqing

AU - Geng, Zhengying

AU - Levesque, Crystal L.

AU - Johnston, Lee J.

AU - Wang, Chunlin

AU - Liu, Ling

AU - Zhang, Jie

AU - Ma, Ning

AU - Qiao, Shiyan

AU - Ma, Xi

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AB - Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.

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KW - Dietary fiber

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