Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs

Ping Liu, Jinbiao Zhao, Pingting Guo, Wenqing Lu, Zhengying Geng, Crystal L. Levesque, Lee J. Johnston, Chunlin Wang, Ling Liu, Jie Zhang, Ning Ma, Shiyan Qiao, Xi Ma

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33 Scopus citations

Abstract

Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.

Original languageEnglish (US)
Article number526
JournalFrontiers in Cellular and Infection Microbiology
Volume7
Issue numberDEC
DOIs
StatePublished - Dec 22 2017

Bibliographical note

Funding Information:
We thank Dr. Xiangli Sun, Zhixiang Sun and Yao Li in Ministry of Agriculture Feed Industry Centre, for the excellent assistance in chemical analysis. This work was supported by the National Key R&D Program of China (2017YFD0500501), College of Animal Science and Technology -Young Talents Cultivation Program- in China Agricultural University (2017DKA001), the National Natural Science Foundation of China (31722054, 31472101, and 31528018), the 111 Project (B16044), the National Department Public Benefit Research Foundation (201403047) and the developmental fund for animal science by Shenzhen Jinxinnong Feed Co., Ltd.

Keywords

  • Bacillus subtilis
  • Cellulolytic bacteria
  • Corn bran
  • Dietary fiber
  • Fermentation
  • Gut microbiota

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