Meningitis remains a worldwide problem, and rapid diagnosis is essential to optimize survival. We evaluated the utility of a multiplex PCR test in differentiating possible etiologies of meningitis. Cerebrospinal fluid (CSF) from 69 HIV-infected Ugandan adults with meningitis was collected at diagnosis (n = 51) and among persons with cryptococcal meningitis during therapeutic lumbar punctures (n = 68). Cryopreserved CSF specimens were analyzed with BioFire FilmArray® Meningitis/Encephalitis panel, which targets 17 pathogens. The panel detected Cryptococcus in the CSF of patients diagnosed with a first episode of cryptococcal meningitis by fungal culture with 100% sensitivity and specificity and differentiated between fungal relapse and paradoxical immune reconstitution inflammatory syndrome in recurrent episodes. A negative FilmArray result was predictive of CSF sterility on follow-up lumbar punctures for cryptococcal meningitis. EBV was frequently detected in this immunosuppressed population (n = 45). Other pathogens detected included: cytomegalovirus (n = 2), varicella zoster virus (n = 2), human herpes virus 6 (n = 1), and Streptococcus pneumoniae (n = 1). The FilmArray Meningitis/Encephalitis panel offers a promising platform for rapid meningitis diagnosis.
|Original language||English (US)|
|Number of pages||6|
|Journal||Diagnostic Microbiology and Infectious Disease|
|State||Published - Mar 1 2016|
Bibliographical noteFunding Information:
Research support for aspects of this project was received from the National Institutes of Allergy and Infectious Disease, the National Institute of Neurologic Disorders and Stroke, and the Fogarty International Center of the National Institutes of Health (R01NS086312, U01AI089244, T32AI055433, R25TW009345).
- Cryptococcal meningitis