Diagnostic accuracy of a novel lateral flow assay for histoplasmosis

Megan Burrows; Janice Miller; Rachael M. Liesman; Nathan C. Bahr

doi:10.1093/mmy/myae051

Diagnostic accuracy of a novel lateral flow assay for histoplasmosis

Megan Burrows, Janice Miller, Rachael M. Liesman, Nathan C. Bahr

Medicine - Infectious Disease and International Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Antigen testing is an important diagnostic tool for histoplasmosis but has limited availability globally. We evaluated the OIDx urine lateral flow antigen assay among 204 persons suspected to have histoplasmosis. Among patients with proven histoplasmosis, sensitivity was 33.3% (3/9, 95% CI 7.5%–70.1%) and specificity 80.5% (157/195, 95% CI 74.3%–85.8%). The MiraVista urine antigen test had better specificity (96.9%) and equal sensitivity. The OIDx test demonstrated 33.3% (3/9) positive agreement and 84.0% (163/194) negative agreement with the MiraVista test. These results should be considered in the context of our low HIV prevalence population with a mixture of pulmonary and disseminated disease.

Original language	English (US)
Article number	myae051
Journal	Medical mycology
Volume	62
Issue number	7
DOIs	https://doi.org/10.1093/mmy/myae051
State	Published - Jul 1 2024

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© The Author(s) 2024. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved.

Keywords

Histoplasma capsulatum
diagnostic test
endemic mycoses
histoplasmosis
lateral flow assay

PubMed: MeSH publication types

Journal Article
Evaluation Study

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10.1093/mmy/myae051

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abstract = "Antigen testing is an important diagnostic tool for histoplasmosis but has limited availability globally. We evaluated the OIDx urine lateral flow antigen assay among 204 persons suspected to have histoplasmosis. Among patients with proven histoplasmosis, sensitivity was 33.3% (3/9, 95% CI 7.5%–70.1%) and specificity 80.5% (157/195, 95% CI 74.3%–85.8%). The MiraVista urine antigen test had better specificity (96.9%) and equal sensitivity. The OIDx test demonstrated 33.3% (3/9) positive agreement and 84.0% (163/194) negative agreement with the MiraVista test. These results should be considered in the context of our low HIV prevalence population with a mixture of pulmonary and disseminated disease.",

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AB - Antigen testing is an important diagnostic tool for histoplasmosis but has limited availability globally. We evaluated the OIDx urine lateral flow antigen assay among 204 persons suspected to have histoplasmosis. Among patients with proven histoplasmosis, sensitivity was 33.3% (3/9, 95% CI 7.5%–70.1%) and specificity 80.5% (157/195, 95% CI 74.3%–85.8%). The MiraVista urine antigen test had better specificity (96.9%) and equal sensitivity. The OIDx test demonstrated 33.3% (3/9) positive agreement and 84.0% (163/194) negative agreement with the MiraVista test. These results should be considered in the context of our low HIV prevalence population with a mixture of pulmonary and disseminated disease.

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Diagnostic accuracy of a novel lateral flow assay for histoplasmosis

Abstract

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Keywords

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