Abstract
The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 171-178 |
| Number of pages | 8 |
| Journal | Developmental and Comparative Immunology |
| Volume | 28 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2004 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the J.G. Salsbury endowment and the USDA National Research Initiative, Cooperative State Research, Education, and Extension Service grant (agreement number 99-35204-7681). We would like to thank Rachel Derscheid, Erin Costello and Jim Fosse for technical assistance.
Keywords
- Antimicrobial peptide
- Fetus
- Follicle-associated epithelium
- Intestine
- Neonate
- Ontogeny
- Sheep β-defensin-2
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