Developmental expression and distribution of sheep β-defensin-2

David K. Meyerholz; Jack M. Gallup; Branka M. Grubor; Richard B. Evans; Brian F. Tack; Paul B. McCray; Mark R. Ackermann

doi:10.1016/S0145-305X(03)00105-8

Developmental expression and distribution of sheep β-defensin-2

David K. Meyerholz, Jack M. Gallup, Branka M. Grubor, Richard B. Evans, Brian F. Tack, Paul B. McCray, Mark R. Ackermann

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.

Original language	English (US)
Pages (from-to)	171-178
Number of pages	8
Journal	Developmental and Comparative Immunology
Volume	28
Issue number	2
DOIs	https://doi.org/10.1016/S0145-305X(03)00105-8
State	Published - Feb 2004
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the J.G. Salsbury endowment and the USDA National Research Initiative, Cooperative State Research, Education, and Extension Service grant (agreement number 99-35204-7681). We would like to thank Rachel Derscheid, Erin Costello and Jim Fosse for technical assistance.

Keywords

Antimicrobial peptide
Fetus
Follicle-associated epithelium
Intestine
Neonate
Ontogeny
Sheep β-defensin-2

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10.1016/S0145-305X(03)00105-8

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title = "Developmental expression and distribution of sheep β-defensin-2",

abstract = "The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.",

keywords = "Antimicrobial peptide, Fetus, Follicle-associated epithelium, Intestine, Neonate, Ontogeny, Sheep β-defensin-2",

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note = "Funding Information: This work was supported by the J.G. Salsbury endowment and the USDA National Research Initiative, Cooperative State Research, Education, and Extension Service grant (agreement number 99-35204-7681). We would like to thank Rachel Derscheid, Erin Costello and Jim Fosse for technical assistance.",

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AU - Evans, Richard B.

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AU - McCray, Paul B.

AU - Ackermann, Mark R.

N1 - Funding Information: This work was supported by the J.G. Salsbury endowment and the USDA National Research Initiative, Cooperative State Research, Education, and Extension Service grant (agreement number 99-35204-7681). We would like to thank Rachel Derscheid, Erin Costello and Jim Fosse for technical assistance.

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N2 - The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.

AB - The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.

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Developmental expression and distribution of sheep β-defensin-2

Abstract

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Keywords

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