Development of the intestinal RNA virus community of healthy broiler chickens

Jigna D. Shah, Prerak T. Desai, Ying Zhang, Sarah K. Scharber, Joshua A Baller, Zheng Xing, Carol J Cardona

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several RNA viruses such as astrovirus, rotavirus, reovirus and parvovirus have been detected in both healthy and diseased commercial poultry flocks. The aim of this study was to characterize (a) the development of the RNA viral community in the small intestines of healthy broiler chickens from hatch through 6 weeks of age (market age) and (b) the contribution of the breeder source vs. bird age in development of the community structure. Intestinal tissue samples were harvested from breeders and their progeny, processed for viral RNA extraction and sequenced using Illumina Hiseq sequencing technology resulting in 100 bp PE reads. The results from this study indicated that the breeder source influenced the RNA viral community only at hatch but later environment i.e. bird age had the more significant effect. The most abundant RNA viral family detected at 2,4 and 6 weeks of age was Astroviridae, which decreased in abundance with age while the abundance of Picornaviridae increased with age.

Original languageEnglish (US)
Article numbere0150094
JournalPloS one
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

RNA Viruses
Viral RNA
Viruses
Chickens
broiler chickens
RNA
Hatches
Birds
Astroviridae
Poultry Diseases
Picornaviridae
Parvovirus
Poultry
Rotavirus
Small Intestine
Protoparvovirus
Tissue
Reoviridae
Technology
birds

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Development of the intestinal RNA virus community of healthy broiler chickens. / Shah, Jigna D.; Desai, Prerak T.; Zhang, Ying; Scharber, Sarah K.; Baller, Joshua A; Xing, Zheng; Cardona, Carol J.

In: PloS one, Vol. 11, No. 2, e0150094, 01.02.2016.

Research output: Contribution to journalArticle

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