TY - JOUR
T1 - Development of the cellulolytic fungus Trichoderma reesei strain with enhanced β-glucosidase and filter paper activity using strong artifical cellobiohydrolase 1 promoter
AU - Zhang, Jiwei
AU - Zhong, Yaohua
AU - Zhao, Xuena
AU - Wang, Tianhong
PY - 2010/12/1
Y1 - 2010/12/1
N2 - To improve the β-glucosidase yield and total cellulase activity of Trichoderma reesei, extracellular β-glucosidase (BGLI) was overexpressed under the control of the modified four-copy cbh1 promoter. Three transformants B2, B12 and B15 with successful integration of the bgl1 gene expression cassette were obtained, which exhibited 3.7-, 2.0- and 1.8-fold increase in β-glucosidase activity than the parental strain RUT C30, respectively. The filter paper activities of the productive transformants B12 and B15 were improved by up to 130% and 55%, respectively. Saccharification of corncob residue with the crude enzyme dosages showed that the reducing sugar yields of B12 (5.59. mg/ml) and B15 (4.80. mg/ml) were 29% and 11% higher than that of RUT C30 (4.34. mg/ml), respectively. The present results proved that the modified four-copy cbh1 promoter was a useful tool for improving the cellulase activity of T. reesei, and the engineering strains developed in this study could be potentially used as promising cell factories for β-glucosidase or cellulase production.
AB - To improve the β-glucosidase yield and total cellulase activity of Trichoderma reesei, extracellular β-glucosidase (BGLI) was overexpressed under the control of the modified four-copy cbh1 promoter. Three transformants B2, B12 and B15 with successful integration of the bgl1 gene expression cassette were obtained, which exhibited 3.7-, 2.0- and 1.8-fold increase in β-glucosidase activity than the parental strain RUT C30, respectively. The filter paper activities of the productive transformants B12 and B15 were improved by up to 130% and 55%, respectively. Saccharification of corncob residue with the crude enzyme dosages showed that the reducing sugar yields of B12 (5.59. mg/ml) and B15 (4.80. mg/ml) were 29% and 11% higher than that of RUT C30 (4.34. mg/ml), respectively. The present results proved that the modified four-copy cbh1 promoter was a useful tool for improving the cellulase activity of T. reesei, and the engineering strains developed in this study could be potentially used as promising cell factories for β-glucosidase or cellulase production.
KW - Cellulase
KW - Modified four-copy cbh1 promoter
KW - Trichoderma reesei
KW - β-Glucosidase 1
UR - http://www.scopus.com/inward/record.url?scp=77956171521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956171521&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.07.078
DO - 10.1016/j.biortech.2010.07.078
M3 - Article
C2 - 20708927
AN - SCOPUS:77956171521
VL - 101
SP - 9815
EP - 9818
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 24
ER -