Development of self-assembling peptide nanovesicle with bilayers for enhanced EGFR-targeted drug and gene delivery

Xiaofei Liang, Bizhi Shi, Kai Wang, Mingliang Fan, Dejin Jiao, Junping Ao, Na Song, Chun Wang, Jianren Gu, Zonghai Li

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Development of rational vectors for efficient drug and gene delivery is crucial for cancer treatment. In this study, epidermal growth factor receptor (EGFR)-binding peptide amphiphile (PA) were used as the primary bilayer skeleton material to construct ultra-stable self-assembling peptide nanovesicle (SPV). The resulted EGFR-targeted SPV (ESPV) could efficiently encapsulate therapeutic cargos (drugs or small interfering RNAs [siRNAs]) or labelled fluorescent cargo (quantum dots [QDs]) and exhibited excellent affinity for EGFR-positive cancer cells. Moreover, ESPV could deliver more drug or plasmid DNA to tumour sites and promote gene expression (a three-fold ratio of ESPVs vs cationic liposomes). Notably, the individual delivery or co-delivery of doxorubicin (DOX) and the acetylcholinesterase (AChE) gene via the ESPVs resulted in excellent drug/gene delivery both in vitro and in vivo and exerted a significant growth-suppressing effect on a liver cancer xenograft. This nanoscale, targeted cargo-packaging technology may provide a new strategy for the design of highly targeted cancer therapy vectors.

Original languageEnglish (US)
Pages (from-to)194-207
Number of pages14
JournalBiomaterials
Volume82
DOIs
StatePublished - Mar 1 2016

Bibliographical note

Funding Information:
This work was supported by The National Natural Science Foundation of China (No. 81572973 ), the small and medium-sized enterprise innovation fund of Shanghai (No. 1302H159400), the Biological Medicine Industry-University-research Project of Shanghai (Grant no. 12DZ1941702 ), the Supporting Program of the “Twelfth Five-year Plan” for Science and Technology Research of China (No. 2012ZX09103-301-006 ), National Basic Research Program (No. 2010CB529902 ), the Biological Medicine Industry-University-research Project of Shanghai (No. 12DZ1941702 ) and The National Natural Science Foundation of China (No. 81302708 ). We thank the Instrumental Analysis Center of Shanghai Jiao Tong University for materials testing.

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

  • Cancer therapy
  • Gene and drug delivery
  • Liposome
  • Peptide
  • Tumour-targeted nanocarrier

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