Development of scalable culture systems for human embryonic stem cells

Samira M. Azarin, Sean P. Palecek

Research output: Contribution to journalReview articlepeer-review

45 Scopus citations


The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.

Original languageEnglish (US)
Pages (from-to)378-384
Number of pages7
JournalBiochemical Engineering Journal
Issue number3
StatePublished - Feb 15 2010


  • Bioreactors
  • Culture scale-up
  • Human embryonic stem cells

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