Development of scalable culture systems for human embryonic stem cells

Samira M. Azarin; Sean P. Palecek

doi:10.1016/j.bej.2009.10.020

Development of scalable culture systems for human embryonic stem cells

Samira M. Azarin, Sean P. Palecek

Chemical Engineering and Materials Science

Research output: Contribution to journal › Review article › peer-review

46 Scopus citations

Abstract

The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.

Original language	English (US)
Pages (from-to)	378-384
Number of pages	7
Journal	Biochemical Engineering Journal
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2009.10.020
State	Published - Feb 15 2010

Bibliographical note

Funding Information:
The authors thank Dr. Ying Nie for the images shown in Fig. 2 . Support was provided by a National Science Foundation Graduate Fellowship to SMA, National Science Foundation grant EFRI-0735903 , and National Institute of Biomedical Imaging and Bioengineering grant R01EB007534 .

Keywords

Bioreactors
Culture scale-up
Human embryonic stem cells

Publisher link

10.1016/j.bej.2009.10.020

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abstract = "The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.",

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note = "Funding Information: The authors thank Dr. Ying Nie for the images shown in Fig. 2 . Support was provided by a National Science Foundation Graduate Fellowship to SMA, National Science Foundation grant EFRI-0735903 , and National Institute of Biomedical Imaging and Bioengineering grant R01EB007534 .",

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AB - The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.

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Development of scalable culture systems for human embryonic stem cells

Abstract

Bibliographical note

Keywords

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