TY - JOUR
T1 - Development of scalable culture systems for human embryonic stem cells
AU - Azarin, Samira M.
AU - Palecek, Sean P.
PY - 2010/2/15
Y1 - 2010/2/15
N2 - The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.
AB - The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.
KW - Bioreactors
KW - Culture scale-up
KW - Human embryonic stem cells
UR - http://www.scopus.com/inward/record.url?scp=75349085710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75349085710&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2009.10.020
DO - 10.1016/j.bej.2009.10.020
M3 - Review article
AN - SCOPUS:75349085710
VL - 48
SP - 378
EP - 384
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 3
ER -