Development of polymerase chain reaction-based assays for bacterial gene detection

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47 Scopus citations


Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR- based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided. (C) 2000 Published by Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalJournal of Microbiological Methods
Issue number3
StatePublished - Aug 2000
Externally publishedYes


  • Polymerase chain reaction (PCR)
  • Primer design
  • Review


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