Development of mPing-based activation tags for crop insertional mutagenesis

Alexander Johnson; Edward Mcassey; Stephanie Diaz; Jacob Reagin; Priscilla S. Redd; Daymond R. Parrilla; Hanh Nguyen; Adrian Stec; Lauren A.L. McDaniel; Thomas E. Clemente; Robert M. Stupar; Wayne A. Parrott; C. Nathan Hancock

doi:10.1002/pld3.300

Development of mPing-based activation tags for crop insertional mutagenesis

Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. McDaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

Original language	English (US)
Article number	e00300
Journal	Plant Direct
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/pld3.300
State	Published - Jan 1 2021

Bibliographical note

Funding Information:
This study was supported by NSF Plant Genome Research Project Grant # 0820769, 1127083, and 1444581. We would like to thank Gary Stacey and Minviluz Stacey (University of Missouri ? Columbia) for their critical feedback on this project. We would also like to thank Theresa McManimon for performing the BertMN01 transformation. Funding was provided by NSF Plant Genome Research Program Grants # 0820769, 1127083, and 1444581.

Funding Information:
We would like to thank Gary Stacey and Minviluz Stacey (University of Missouri – Columbia) for their critical feedback on this project. We would also like to thank Theresa McManimon for performing the BertMN01 transformation. Funding was provided by NSF Plant Genome Research Program Grants # 0820769, 1127083, and 1444581.

Publisher Copyright:
© 2021 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

Keywords

activation tagging
gene discovery
soybean
transposable element

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10.1002/pld3.300

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abstract = "Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.",

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AU - Nguyen, Hanh

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Development of mPing-based activation tags for crop insertional mutagenesis

Abstract

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