Development of Microsatellite Markers for the Apomictic Triploid Fern Myriopteris lindheimeri (Pteridaceae)

Amanda L. Grusz, Kathleen M. Pryer

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Premise of the study: Microsatellite markers were developed for investigating the population dynamics of Myriopteris lindheimeri (Pteridaceae), an apomictic triploid fern endemic to deserts of the southwestern United States and Mexico. Methods and Results: Using 454 sequencing, 21 microsatellite markers were developed. Of these, 14 were polymorphic with up to five alleles per locus and eight markers amplified in one or more congeneric close relatives (M. covillei, M. fendleri, M. aurea, and M. rufa). To demonstrate marker utility, M. lindheimeri samples from three Arizona populations were genotyped at nine loci. For each population, diversity measures including percent polymorphic loci, frequency of heterozygotes across all loci, and genotypic diversity were calculated. Across the three populations, on average, 63% of loci were polymorphic, the average frequency of heterozygotes (across all loci) was 0.32, and average genotypic diversity was 0.34. Conclusions: These markers provide a foundation for future studies exploring polyploidy and apomixis in myriopterid ferns.

Original languageEnglish (US)
Article number1500061
JournalApplications in Plant Sciences
Volume3
Issue number11
DOIs
StatePublished - Nov 1 2015

Keywords

  • Myriopteris lindheimeri
  • asexual
  • cheilanthoid
  • polyploidy
  • pteridophyte
  • simple sequence repeats (SSRs)

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