Development of larvae of Ascaris suum from the third to the fourth stage in a chemically defined medium

B. E. Stromberg; P. B. Khoury; E. J.L. Soulsby

doi:10.1016/0020-7519(77)90083-2

Development of larvae of Ascaris suum from the third to the fourth stage in a chemically defined medium

B. E. Stromberg, P. B. Khoury, E. J.L. Soulsby

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Third stage larvae of Ascaris suum, recovered from the lungs of rabbits 7 days after oral infection with eggs, were cultured to the fourth larval stage in chemically defined, low molecular weight medium. The medium consisted of tissue culture medium 199, supplemented with glucose and glycyl-hystidyl-lysine and gassed with a mixture of N₂-CO₂-O₂ (90:5:5). Growth and development in this medium were similar to that in media supplemented with whole serum or with a serum dialysate.

Original language	English (US)
Pages (from-to)	149-151
Number of pages	3
Journal	International Journal for Parasitology
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/0020-7519(77)90083-2
State	Published - Apr 1977

Keywords

Ascaris suum
chemically defined medium
glycyl-histidyl-lysine
in vitro culture

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10.1016/0020-7519(77)90083-2

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title = "Development of larvae of Ascaris suum from the third to the fourth stage in a chemically defined medium",

abstract = "Third stage larvae of Ascaris suum, recovered from the lungs of rabbits 7 days after oral infection with eggs, were cultured to the fourth larval stage in chemically defined, low molecular weight medium. The medium consisted of tissue culture medium 199, supplemented with glucose and glycyl-hystidyl-lysine and gassed with a mixture of N2-CO2-O2 (90:5:5). Growth and development in this medium were similar to that in media supplemented with whole serum or with a serum dialysate.",

keywords = "Ascaris suum, chemically defined medium, glycyl-histidyl-lysine, in vitro culture",

author = "Stromberg, {B. E.} and Khoury, {P. B.} and Soulsby, {E. J.L.}",

year = "1977",

month = apr,

doi = "10.1016/0020-7519(77)90083-2",

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AU - Stromberg, B. E.

AU - Khoury, P. B.

AU - Soulsby, E. J.L.

PY - 1977/4

Y1 - 1977/4

N2 - Third stage larvae of Ascaris suum, recovered from the lungs of rabbits 7 days after oral infection with eggs, were cultured to the fourth larval stage in chemically defined, low molecular weight medium. The medium consisted of tissue culture medium 199, supplemented with glucose and glycyl-hystidyl-lysine and gassed with a mixture of N2-CO2-O2 (90:5:5). Growth and development in this medium were similar to that in media supplemented with whole serum or with a serum dialysate.

AB - Third stage larvae of Ascaris suum, recovered from the lungs of rabbits 7 days after oral infection with eggs, were cultured to the fourth larval stage in chemically defined, low molecular weight medium. The medium consisted of tissue culture medium 199, supplemented with glucose and glycyl-hystidyl-lysine and gassed with a mixture of N2-CO2-O2 (90:5:5). Growth and development in this medium were similar to that in media supplemented with whole serum or with a serum dialysate.

KW - Ascaris suum

KW - chemically defined medium

KW - glycyl-histidyl-lysine

KW - in vitro culture

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Development of larvae of Ascaris suum from the third to the fourth stage in a chemically defined medium

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