Development of IL-22-producing NK lineage cells from umbilical cord blood hematopoietic stem cells in the absence of secondary lymphoid tissue

Qin Tang, Yong Oon Ahn, Peter Southern, Bruce R. Blazar, Jeffery S. Miller, Michael R. Verneris

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Human secondary lymphoid tissues (SLTs) contain interleukin-22 (IL-22)-producing cells with an immature NK phenotype. Given their location, these cells are difficult to study. We have generated large numbers of NK22 cells from hematopoietic stem cells. HSC-derived NK22 cells show a CD56 + CD117highCD94- phenotype, consistent with stage III NK progenitors. Like freshly isolated SLT stage III cells, HSC-derived NK22 cells express NKp44, CD161, CCR6, IL1 receptor, AHR, and ROR-γτ. IL-1β and IL-23 stimulation results in significant IL-22 but not interferon-γ production. Supernatant from these cells increases CD54 expression on mesenchymal stem cells. Thus, IL-22-producing NK cells can be generated in the absence of SLT. HSC-derived NK22 cells will be valuable in understanding this rare NK subset and create the opportunity for human translational clinical trials.

Original languageEnglish (US)
Pages (from-to)4052-4055
Number of pages4
JournalBlood
Volume117
Issue number15
DOIs
StatePublished - Apr 14 2011

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