Development of Genome-Driven, Lifestyle-Informed Markers for Identification of the Cereal-Infecting Pathogens Xanthomonas translucens Pathovars undulosa and translucens

Veronica Roman-Reyna, Rebecca D. Curland, Yesenia Velez-Negron, Kristi E Ledman, Diego E. Gutierrez-Castillo, Jonathan Beutler, Jules Butchacas, Gurcharn Singh Brar, Robyn Roberts, Ruth Dill-Macky, Jonathan M. Jacobs

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Bacterial leaf streak, bacterial blight, and black chaff caused by Xanthomonas translucens pathovars are major diseases affecting small grains. Xanthomonas translucens pv. translucens and X. translucens pv. undulosa are seedborne pathogens that cause similar symptoms on barley, but only X. translucens pv. undulosa causes bacterial leaf streak of wheat. Recent outbreaks of X. translucens have been a concern for wheat and barley growers in the Northern Great Plains; however, there are limited diagnostic tools for pathovar differentiation. We developed a multiplex PCR based on whole-genome differences to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. We validated the primers across different Xanthomonas and non-Xanthomonas strains. To our knowledge, this is the first multiplex PCR to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. These molecular tools will support disease management strategies enabling detection and pathovar incidence analysis of X. translucens.

Original languageEnglish (US)
Pages (from-to)2110-2118
Number of pages9
JournalPhytopathology
Volume113
Issue number11
DOIs
StatePublished - Nov 2023

Bibliographical note

Publisher Copyright:
© 2023 American Phytopathological Society. All rights reserved.

Keywords

  • bacterial pathogens
  • bioinformatics
  • evolution
  • genomics
  • pathogen detection
  • techniques

PubMed: MeSH publication types

  • Journal Article

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