Development of Ascaris suum larvae from the third to fourth stage, in vitro

Stephen R. Sylk, B. E. Stromberg, E. J.L. Soulsby

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Larvae of Ascaris suum recovered from the lungs of experimentally infected guinea pigs or rabbits, seven to ten days after infection, underwent the third moult in vitro to become fourth-stage larvae. Ecdysis was first observed on the first and second day of culture and was completed by the eighth day. The culture medium consisted of tissue culture Medium 199 supplemented with either guinea pig or porcine serum and glucose in a gaseous atmosphere of Nitrogen:Oxygen:Carbon dioxide (90:5:5). By the twelfth day in culture, early development of both the male and female reproductive systems was observed.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalInternational Journal for Parasitology
Issue number3
StatePublished - Jun 1974


  • Ascaris sum
  • in vitro culture


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