Development of an ion-channel sensor for heparin detection

Victor P.Y. Gadzekpo, Philippe Bühlmann, Kang Ping Xiao, Hiroshi Aoki, Yoshio Umezawa

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


The ion-channel sensor technique was used to determine heparin concentrations in artificial and horse serum with cyclic voltammetry. The sensor is based on self-assembled monolayers (SAMs) of thioctic acid on which protamine is attached as a receptor to control the rate of [Mo(CN)8]4- oxidation or [Fe(CN)6]3- reduction in the presence of heparin. The analyte, heparin, with its negative charges, neutralizes the positive charges on the protamine receptor and at high heparin concentrations provides the electrode surface with an excess of negative charge, thereby repulsing the marker ions from the electrode surface. This decreases the redox currents and makes them a function of the analyte concentration. In artificial serum, a linear concentration range of 0.05-1.5μg/ml was obtained for the heparin response at a scan rate of 10.24V/s when [Mo(CN)8]4- was used as marker. Repeated measurements of heparin in artificial and horse serum gave average heparin concentrations of 1.30 and 1.56μg/ml, respectively, compared to 1.25μg/ml heparin that was introduced into the serum. Measurements of heparin in horse serum using a fresh electrode for each sample, however, gave an average heparin concentration of 1.21μg/ml with a standard deviation of 0.026μg/ml. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)163-173
Number of pages11
JournalAnalytica Chimica Acta
Issue number1-2
StatePublished - May 1 2000

Bibliographical note

Funding Information:
Y.U. acknowledges the Japan Society for the Promotion of Science (JSPS) for the Invitation Fellowship Programme for Research in Japan (long term) to V.P.Y.G. A JSPS fellowship to K.P.X. is also gratefully acknowledged. We thank Daiichi Chemical Company, Tokyo, Japan and Tissue Culture Biologicals, Tokyo, Japan for the kind donation of heparin and horse serum, respectively. This work was supported by Grant-in-Aid for Scientific Research for the Priority Areas of ‘Electrochemistry of Ordered Interfaces’ (No. 10131216).

Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.


  • Heparin
  • Ion-channel sensor
  • Polyion
  • Protamine
  • Self-assembled monolayer
  • Thioctic acid
  • Voltammetry


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