Bovine viral diarrhea virus (BVDV) is an important cause of economic losses world-wide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demon-strating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle.
|Translated title of the contribution||Development of an enhanced bovine viral diarrhea virus subunitvaccine based on E2 glycoprotein fused to a single chain antibodywhich targets to antigen-presenting cells|
|Number of pages||5|
|Journal||Revista Argentina de Microbiologia|
|State||Published - 2015|
Bibliographical noteFunding Information:
The authors are grateful to Dr. Osvaldo Zabal and his group for providing valuable help on cell culture and to Dr. Oscar Taboga and Dr. Gabriela Lopez for assistance in protein expression. Financial support was received through INTA's Specific Project AEGR 2413 .
© 2014 Asociación Argentina de Microbiología.
- Bovine viral diarrheavirus
- E2 glycoprotein
- Single chainantibody
- Subunit vaccine