Development of a whole blood lymphocyte stimulation assay for detecting hypersensitivity to PPD in cattle experimentally infected with Mycobacterium bovis

C. C. Muscoplat, D. W. Johnson, C. O. Thoen, M. D. Ayivor, D. J. Klausner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A whole blood lymphocyte stimulation assay utilizing the uptake of tritiated thymidine was developed for the detection of Mycobacterium bovis sensitivity in cattle. Results on eight M. bovis infected animals (six to ten weeks after infection) and eight control animals show that satisfactory lymphocyte stimulation can be obtained using heparinized whole blood diluted 10-fold in tissue culture medium and cultured with purified protein derivative (PPD) for three to seven days. Infected animals exhibited significantly greater stimulation when cultured with PPD than did control animals.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalVeterinary Microbiology
Volume2
Issue number3
DOIs
StatePublished - Oct 1977

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