A whole blood lymphocyte stimulation assay utilizing the uptake of tritiated thymidine was developed for the detection of Mycobacterium bovis sensitivity in cattle. Results on eight M. bovis infected animals (six to ten weeks after infection) and eight control animals show that satisfactory lymphocyte stimulation can be obtained using heparinized whole blood diluted 10-fold in tissue culture medium and cultured with purified protein derivative (PPD) for three to seven days. Infected animals exhibited significantly greater stimulation when cultured with PPD than did control animals.
Bibliographical noteFunding Information:
C.C. MUSCOPLAT I, D.W. JOHNSON l. C.O. THOEN 2, M.D. AYIVOR* and D.J. KLAUSNER ! ~Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minn. 55108 (U.S.A.) 2Veterinary Services Laboratories, APHIS, USDA Ames, Iowa 50010 (U.S.A.) *Present address: Ahmadu Bello University, Faculty of Veterinary Medicine, Zaria (Nigeria) This investigation was supported by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Hyattsville, Md (U.S.A.) (Received 3 May 1977)