Development of a novel efficient method to construct an adenovirus library displaying random peptides on the fiber knob

Yuki Yamamoto, Naoko Goto, Kazuki Miura, Kenta Narumi, Shumpei Ohnami, Hiroaki Uchida, Yoshiaki Miura, Masato Yamamoto, Kazunori Aoki

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Redirection of adenovirus vectors by engineering the capsid-coding region has shown limited success because proper targeting ligands are generally unknown. To overcome this limitation, we constructed an adenovirus library displaying random peptides on the fiber knob, and its screening led to successful selections of several particular targeted vectors. In the previous library construction method, the full length of an adenoviral genome was generated by a Cre-lox mediated in vitro recombination between a fiber-modified plasmid library and the enzyme-digested adenoviral DNA/terminal protein complex (DNA-TPC) before transfection to the producer cells. In this system, the procedures were complicated and time-consuming, and approximately 30% of the vectors in the library were defective with no displaying peptide. These may hinder further extensive exploration of cancer-targeting vectors. To resolve these problems, in this study, we developed a novel method with the transfection of a fiber-modified plasmid library and a fiberless adenoviral DNA-TPC in Cre-expressing 293 cells. The use of in-cell Cre recombination and fiberless adenovirus greatly simplified the library-making steps. The fiberless adenovirus was useful in suppressing the expansion of unnecessary adenovirus vectors. In addition, the complexity of the library was more than a 104 level in one well in a 6-well dish, which was 10-fold higher than that of the original method. The results demonstrated that this novel method is useful in producing a high quality live adenovirus library, which could facilitate the development of targeted adenovirus vectors for a variety of applications in medicine.

Original languageEnglish (US)
Pages (from-to)1069-1074
Number of pages6
JournalMolecular pharmaceutics
Volume11
Issue number3
DOIs
StatePublished - Mar 3 2014

Keywords

  • Cre recombination
  • adenovirus
  • fiberless
  • library
  • targeting vector

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