Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers

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21 Scopus citations

Abstract

In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalBiosensors and Bioelectronics
Volume126
DOIs
StatePublished - Feb 1 2019

Bibliographical note

Funding Information:
We acknowledge XPRIZE Foundation and Nokia Sensing XCHALLENGE competition for motivating the design of the z-Lab Diagnosis Platform which won a Distinguished Prize Award. This work was supported in part by an Ovarian Cancer Translational Pilot Award (W81XWH-11-1-0496) through the U.S. Department of Defense. Additional funding came both from the Jan Chorzempa Cancer Research Endowed Fund (11726) though the University of Minnesota Foundation and from a Doctoral Dissertation Fellowship through the University of Minnesota Graduate School. Parts of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network (www.mrfn.org) via the MRSEC program.

Funding Information:
We acknowledge XPRIZE Foundation and Nokia Sensing XCHALLENGE competition for motivating the design of the z-Lab Diagnosis Platform which won a Distinguished Prize Award. This work was supported in part by an Ovarian Cancer Translational Pilot Award (W81XWH-11-1-0496) through the U.S. Department of Defense . Additional funding came both from the Jan Chorzempa Cancer Research Endowed Fund (11726) though the University of Minnesota Foundation and from a Doctoral Dissertation Fellowship through the University of Minnesota Graduate School. Parts of this work were carried out in the Characterization Facility, University of Minnesota , a member of the NSF-funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Biomarkers
  • CA125
  • Giant magnetoresistance
  • HE4
  • Hand-held system
  • IL6
  • Multiplex detection
  • Ovarian cancer

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