Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalBiosensors and Bioelectronics
Volume126
DOIs
StatePublished - Feb 1 2019

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Biosensing Techniques
Tumor Biomarkers
Computer programming
Biosensors
Ovarian Neoplasms
Assays
Nanomagnetics
CA-125 Antigen
Computer-Assisted Signal Processing
Proteins
Magnetoelectronics
Firmware
Epididymis
Smartphones
Bluetooth
Biomarkers
Antigens
Digital signal processing
User interfaces
Limit of Detection

Keywords

  • Biomarkers
  • CA125
  • Giant magnetoresistance
  • HE4
  • Hand-held system
  • IL6
  • Multiplex detection
  • Ovarian cancer

PubMed: MeSH publication types

  • Journal Article

Cite this

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title = "Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers",
abstract = "In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.",
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author = "Todd Klein and Wei Wang and Lina Yu and Kai Wu and Boylan, {Kristin L} and Vogel, {Rachel I} and Skubitz, {Amy P} and Jianping Wang",
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AU - Wang, Wei

AU - Yu, Lina

AU - Wu, Kai

AU - Boylan, Kristin L

AU - Vogel, Rachel I

AU - Skubitz, Amy P

AU - Wang, Jianping

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AB - In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.

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