Development of a LC-MS/MS method for urinary hydroxytyrosol as a marker for consumption of olive oil

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Abstract

The aim of this study was to develop and validate a LC-MS/MS method to quantitate hydroxytyrosol in urine for clinical studies to monitor compliance of consumption of dietary extra virgin olive oil. Both pre-analytical and analytical issues were examined. The stability of hydroxytyrosol was monitored in both urine and synthetic urine at four different temperatures (room temperature, 4 °C, −20 °C, or −80 °C) for four weeks either with or without the additive acetic acid. The LC-MS/MS method was validated by performing linearity, imprecision, recovery, and carryover studies.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalClinical Mass Spectrometry
Volume15
DOIs
StatePublished - Jan 2020

Bibliographical note

Funding Information:
Dr. Seegmiller has nothing to disclose. Dr. Johnson has nothing to disclose. Dr. Killeen reports grants from NIH, outside the submitted work.

Publisher Copyright:
© 2019

Keywords

  • Hydroxytyrosol
  • LC-MS/MS
  • Mediterranean diet
  • Olive oil
  • Stability
  • Urine

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