Development of a LC-MS/MS method for urinary hydroxytyrosol as a marker for consumption of olive oil

Research output: Contribution to journalArticle

Abstract

The aim of this study was to develop and validate a LC-MS/MS method to quantitate hydroxytyrosol in urine for clinical studies to monitor compliance of consumption of dietary extra virgin olive oil. Both pre-analytical and analytical issues were examined. The stability of hydroxytyrosol was monitored in both urine and synthetic urine at four different temperatures (room temperature, 4 °C, −20 °C, or −80 °C) for four weeks either with or without the additive acetic acid. The LC-MS/MS method was validated by performing linearity, imprecision, recovery, and carryover studies.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalClinical Mass Spectrometry
Volume15
DOIs
StatePublished - Jan 2020

Fingerprint

Acetic Acid
Recovery
Temperature
3,4-dihydroxyphenylethanol
Olive Oil
Compliance

Keywords

  • Hydroxytyrosol
  • LC-MS/MS
  • Mediterranean diet
  • Olive oil
  • Stability
  • Urine

Cite this

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title = "Development of a LC-MS/MS method for urinary hydroxytyrosol as a marker for consumption of olive oil",
abstract = "The aim of this study was to develop and validate a LC-MS/MS method to quantitate hydroxytyrosol in urine for clinical studies to monitor compliance of consumption of dietary extra virgin olive oil. Both pre-analytical and analytical issues were examined. The stability of hydroxytyrosol was monitored in both urine and synthetic urine at four different temperatures (room temperature, 4 °C, −20 °C, or −80 °C) for four weeks either with or without the additive acetic acid. The LC-MS/MS method was validated by performing linearity, imprecision, recovery, and carryover studies.",
keywords = "Hydroxytyrosol, LC-MS/MS, Mediterranean diet, Olive oil, Stability, Urine",
author = "Johnson, {Lisa M.} and Killeen, {Anthony A.} and Seegmiller, {Jesse C.}",
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AB - The aim of this study was to develop and validate a LC-MS/MS method to quantitate hydroxytyrosol in urine for clinical studies to monitor compliance of consumption of dietary extra virgin olive oil. Both pre-analytical and analytical issues were examined. The stability of hydroxytyrosol was monitored in both urine and synthetic urine at four different temperatures (room temperature, 4 °C, −20 °C, or −80 °C) for four weeks either with or without the additive acetic acid. The LC-MS/MS method was validated by performing linearity, imprecision, recovery, and carryover studies.

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KW - Mediterranean diet

KW - Olive oil

KW - Stability

KW - Urine

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