Développement d’un homogénat de poumon spécifique de troupeau pour exposition à Mycoplasma hyopneumoniae dans des conditions de terrain

Translated title of the contribution: Development of a herd-specific lung homogenate for exposure to Mycoplasma hyopneumoniae under field conditions

Rebecca C. Robbins, Alyssa M. Betlach, Maria R. MondragonEvans, Maria Pieters

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The swine industry is known for holding high standards of disease control and elimination. However, partial disease control for Mycoplasma hyopneumoniae at the farm level has been evident and has driven initiatives for unconventional health management strategies. Several approaches focused on gilt exposure for M hyopneumoniae using a herdspecific lung homogenate have been performed in the field. Nevertheless, variations in efficacy are apparent and a publicly available protocol for producing M hyopneumoniae lung homogenate under field conditions is not available. In this practice tip, a protocol is described for developing a herdspecific lung homogenate for M hyopneumoniae exposure intended for use in veterinarysupervised elimination or control programs. A herdspecific lung homogenate inoculum, free of secondary respiratory pathogens for the herd of intended use and with an adequate M hyopneumoniae concentration, was obtained through extensive diagnostic testing and evaluation of M hyopneumoniae localization within the lung. Molecular methods were applied to characterize the M hyopneumoniae present in the lung and to evaluate the genomic stability of the bacterium during the exposure process. In doing so, a herdspecific M hyopneumoniae lung homogenate for gilt acclimation was obtained under field conditions.

Translated title of the contributionDevelopment of a herd-specific lung homogenate for exposure to Mycoplasma hyopneumoniae under field conditions
Original languageSpanish
Pages (from-to)221-227
Number of pages7
JournalJournal of Swine Health and Production
Volume27
Issue number4
StatePublished - Jan 1 2019

Keywords

  • Disease control
  • Elimination
  • Gilt acclimation
  • Lung homogenate
  • Mycoplasma hyopneumoniae
  • Swine

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