Community-based organizations (CBOs), particularly minority-based CBOs, are instrumental in the delivery of HIV/AIDS prevention programs and services. Despite a tremendous need, many CBOs lack the capacity to plan, implement, and evaluate targeted prevention interventions to serve culturally diverse populations. This article describes a comprehensive, innovative curriculum for building capacity within CBOs providing HIV prevention programming. An overview of the process through which the Centers for Disease Control and Prevention/ Association of Schools of Public Health Institute for HIV Prevention Leadership evolved is discussed. The development of the curriculum occurred in four phases: needs assessment, curricular design, implementation, and evaluation. A description of the Institute's evaluation strategies and procedures are described. Selected preliminary evaluation results from two cohorts of participants indicate significant knowledge gains and high levels of satisfaction with the Institute sessions, assignments, and faculty. The Institute's model shows promise for the provision of capacity building education at the individual and organizational level for community-based professionals implementing HIV prevention programs.