Polymorphic genetic markers are important tools in the construction of comprehensive genetic maps. This paper reports on the isolation and characterization of 90 new bovine microsatellite (ms) loci from enriched genomic libraries. The sequence of one clone (locus MNB-85) showed significant similarity to an intron of the human promyelocytic leukemia zinc finger protein (PLZF) gene. Screening of bovine and porcine somatic cell panels places the bovine PLZF homolog on BTA-15 and the porcine PLZF homolog on SSC-9. The 90 new microsatellite loci increase the number of microsatellites available for cattle by >5%.