Development of 47 new microsatellite markers from a BTA6 library

  • K. M. Reed
  • , N. Ihara
  • , F. A. Ponce De León
  • , T. S. Sonstegard
  • , T. P.L. Smith
  • , G. L. Bennett
  • , Y. Sugimoto
  • , C. W. Beattie

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalAnimal Biotechnology
Volume13
Issue number2
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
This study was supported by a grant from the USDA-NRICGP, the Japan Livestock Technology Association and the Japan Racing and Livestock Promotion Foundation.

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