Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.
Bibliographical noteFunding Information:
This study was supported by a grant from the USDA-NRICGP, the Japan Livestock Technology Association and the Japan Racing and Livestock Promotion Foundation.
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