Development and validation of a perfect KASP marker for fusarium head blight resistance gene Fhb1 in wheat

Lovepreet Singh, James A. Anderson, Jianli Chen, Bikram S. Gill, Vijay K. Tiwari, Nidhi Rawat

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12 Scopus citations


Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFT-based Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gel-based gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the Fhb1 region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_ KASP is a perfect diagnostic marker for Fhb1 and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.

Original languageEnglish (US)
Pages (from-to)200-207
Number of pages8
JournalPlant Pathology Journal
Issue number3
StatePublished - 2019

Bibliographical note

Funding Information:
This work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1016879 and Maryland Agricultural Experiment Station via MAES Grant No. 2956952.

Publisher Copyright:
© The Korean Society of Plant Pathology.


  • Fhb1
  • Fusarium head blight
  • KASP assay
  • Pore-forming toxin-like (PFT)
  • Wheat


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