Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma

S. M. Illamola; E. Valade; D. Hirt; E. Dulioust; Y. Zheng; J. P. Wolf; J. M. Tréluyer

doi:10.1016/j.jchromb.2016.08.011

Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma

S. M. Illamola, E. Valade, D. Hirt, E. Dulioust, Y. Zheng, J. P. Wolf, J. M. Tréluyer

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Accurate and sensitive liquid-chromatography tandem mass spectrometry method for the quantification of tenofovir and emtricitabine in seminal plasma has been developed and full validated. Molecules were separated by high-performance liquid chromatography on an Atlantis T3 C₁₈ column using a gradient of deionized water and methanol, including 0.05% formic acid (250 μl/min) and detected by electrospray ionisation/tandem mass spectrometry in positive ion mode. The method was validated over a clinical range of 3.13–1000 ng/mL for tenofovir and 6.25–2000 ng/mL for emtricitabine. Inter and intra-assay precisions were <9.37% for tenofovir and<10.88% for emtricitabine, and accuracies were between 0.48% and 8.43% for tenofovir, and between 0.64% and 13.87% for emtricitabine. The developed method was successfully applied for analysing tenofovir and emtricitabine concentrations in seminal plasma samples from a clinical study. The use of tandem mass spectrometry can be a suitable method for the analysis of this kind of matrices, providing high sensitivity and specificity to the analysis.

Original language	English (US)
Pages (from-to)	234-241
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	1033-1034
DOIs	https://doi.org/10.1016/j.jchromb.2016.08.011
State	Published - Oct 15 2016
Externally published	Yes

Bibliographical note

Funding Information:
We acknowledge the French National Agency for Research on AIDS and Viral Hepatitis (ANRS) for sponsoring the EVARIST study. We also acknowledge SIDACTION for the financial support of this work.

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

Emtricitabine
LC–MS/MS
Seminal plasma
Tenofovir

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10.1016/j.jchromb.2016.08.011

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Illamola, S. M., Valade, E., Hirt, D., Dulioust, E., Zheng, Y., Wolf, J. P., & Tréluyer, J. M. (2016). Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1033-1034, 234-241. https://doi.org/10.1016/j.jchromb.2016.08.011

Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma. / Illamola, S. M.; Valade, E.; Hirt, D. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1033-1034, 15.10.2016, p. 234-241.

Research output: Contribution to journal › Article › peer-review

Illamola, SM, Valade, E, Hirt, D, Dulioust, E, Zheng, Y, Wolf, JP & Tréluyer, JM 2016, 'Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1033-1034, pp. 234-241. https://doi.org/10.1016/j.jchromb.2016.08.011

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abstract = "Accurate and sensitive liquid-chromatography tandem mass spectrometry method for the quantification of tenofovir and emtricitabine in seminal plasma has been developed and full validated. Molecules were separated by high-performance liquid chromatography on an Atlantis T3 C18 column using a gradient of deionized water and methanol, including 0.05% formic acid (250 μl/min) and detected by electrospray ionisation/tandem mass spectrometry in positive ion mode. The method was validated over a clinical range of 3.13–1000 ng/mL for tenofovir and 6.25–2000 ng/mL for emtricitabine. Inter and intra-assay precisions were <9.37% for tenofovir and<10.88% for emtricitabine, and accuracies were between 0.48% and 8.43% for tenofovir, and between 0.64% and 13.87% for emtricitabine. The developed method was successfully applied for analysing tenofovir and emtricitabine concentrations in seminal plasma samples from a clinical study. The use of tandem mass spectrometry can be a suitable method for the analysis of this kind of matrices, providing high sensitivity and specificity to the analysis.",

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note = "Funding Information: We acknowledge the French National Agency for Research on AIDS and Viral Hepatitis (ANRS) for sponsoring the EVARIST study. We also acknowledge SIDACTION for the financial support of this work. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

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AU - Dulioust, E.

AU - Zheng, Y.

AU - Wolf, J. P.

AU - Tréluyer, J. M.

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Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma

Abstract

Bibliographical note

Keywords

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