Development and evaluation of an ELISA to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus

Melissa L. Palmer-Densmore, Anthony F. Johnson, Marta I.J. Sabara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A rapid and reproducible enzyme linked immunosorbent assay (ELISA) was developed for detection of canine coronavirus (CCV) specific antibodies directed to both the nucleocapsid (NC) and the spike (s) proteins. The coating antigen, a methanol-treated, S-protein enriched preparation, was produced by subjecting infected cells to Triton X-114 detergent followed by phase separation. The sensitivity of this assay was determined by following the course of infection in dogs experimentally infected with CCV. The specificity of the antibody response was determined by Western blot analysis and supported the increase magnitude of the ELISA response and the presence of serum neutralizing (SN) antibody. Due to the sensitivity and specificity of the IgG response detected by this assay it can be used to determine both virus exposure and vaccine efficacy.

Original languageEnglish (US)
Pages (from-to)1-22
Number of pages22
JournalJournal of Immunoassay
Volume19
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Canine Coronavirus
Nucleocapsid Proteins
Nucleocapsid
Immunosorbents
Antibody Formation
Assays
Enzyme-Linked Immunosorbent Assay
Antibodies
Protein S
Enzymes
Neutralizing Antibodies
Detergents
Methanol
Proteins
Vaccines
Immunoglobulin G
Western Blotting
Dogs
Viruses
Antigens

Keywords

  • CCV
  • Coating antigen preparation
  • Enzyme immunoassay
  • Methanol
  • Triton X- 114

Cite this

Development and evaluation of an ELISA to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus. / Palmer-Densmore, Melissa L.; Johnson, Anthony F.; Sabara, Marta I.J.

In: Journal of Immunoassay, Vol. 19, No. 1, 01.01.1998, p. 1-22.

Research output: Contribution to journalArticle

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